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CD5 antibody

CD5 Reactivity: Rat FACS, IP, IHC (p), IHC (fro) Host: Mouse Monoclonal OX-19 unconjugated
Catalog No. ABIN120388
  • Target See all CD5 Antibodies
    CD5
    Reactivity
    • 222
    • 90
    • 58
    • 27
    • 22
    • 16
    • 15
    • 15
    • 13
    • 12
    • 11
    • 10
    • 5
    • 2
    • 1
    • 1
    • 1
    Rat
    Host
    • 167
    • 81
    • 56
    • 1
    Mouse
    Clonality
    • 233
    • 70
    Monoclonal
    Conjugate
    • 110
    • 38
    • 32
    • 28
    • 13
    • 8
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD5 antibody is un-conjugated
    Application
    • 236
    • 109
    • 68
    • 67
    • 65
    • 53
    • 30
    • 29
    • 22
    • 18
    • 13
    • 13
    • 12
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    SM258PS recognises the rat CD5 cell surface antigen, a 69 kDa glycoprotein expressed by T cells, thymocytes and a subset of B cells. This product is routinely tested in flow cytometry on rat splenocytes.
    Purification
    Protein G chromatography
    Immunogen
    Rat thymocyte glycoproteins.
    Clone
    OX-19
    Isotype
    IgG1
    Top Product
    Discover our top product CD5 Primary Antibody
  • Application Notes
    Flow Cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin embedded sections: OX-19 has been reported as being suitable for use on periodate-lysine paraformaldehyde (PLP) fixed paraffin embedded tissue (4). Immunoprecipitation.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4, with 0.09 % sodium azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    CD5
    Alternative Name
    CD5 (CD5 Products)
    Synonyms
    CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody
    Background
    CD5 is a 55 kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5
    Gene ID
    54236
    NCBI Accession
    NP_062168
    UniProt
    P51882
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