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NF-kB p65 antibody (Nuclear Factor-kB p65) (C-Term) Primary Antibody

NFkBP65 Reactivity: Human, Mouse EMSA, EIA, IHC (fro), IP, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN121887
$1,046.10
Plus shipping costs $45.00
0.1 mL
local_shipping Shipping to: United States
Available
  • Target
    NF-kB p65 (NFkBP65)
    Binding Specificity
    • 36
    • 28
    • 27
    • 27
    • 27
    • 27
    • 23
    • 21
    • 21
    • 19
    • 16
    • 15
    • 15
    • 14
    • 13
    • 11
    • 11
    • 10
    • 8
    • 8
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 546
    • 383
    • 310
    • 49
    • 44
    • 35
    • 17
    • 17
    • 13
    • 11
    • 7
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Human, Mouse
    Host
    • 500
    • 47
    • 6
    • 1
    • 1
    Rabbit
    Clonality
    • 482
    • 71
    Polyclonal
    Conjugate
    • 298
    • 31
    • 22
    • 19
    • 18
    • 17
    • 17
    • 17
    • 17
    • 17
    • 13
    • 10
    • 10
    • 10
    • 10
    • 10
    • 7
    • 7
    • 2
    • 2
    • 1
    This NF-kB p65 antibody is un-conjugated
    Application
    • 353
    • 215
    • 157
    • 116
    • 107
    • 96
    • 92
    • 74
    • 68
    • 29
    • 22
    • 11
    • 10
    • 10
    • 10
    • 7
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
    Specificity
    This antibody is reactive with NFkB p65 subunit (Rel A). A control peptide that competes specifically with the antiserum is available SP2142CP.
    Purification
    Serum
    Immunogen
    NFkB p65 peptide corresponding to the C-terminus region of the human protein conjugated to KLH
  • Application Notes
    ELISA. Immunoprecipitation. Western Blot: 1/2000 - 1/5000. Gel Super Shift Assays: 0.5-1 μL per test, In a gel supershift assay it was found to be active against all p65 containing human and mouse NFkB complexes using 0.1 μL to 1.1 μL per assay.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Containing 0.01 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    NF-kB p65 (NFkBP65)
    Alternative Name
    RELA / NF-kB p65 (NFkBP65 Antibody Abstract)
    Synonyms
    C-Rel, NFKB3, p65, NFkB, c-rel, zgc:100833, Xrel2, Xrel3, rel, rel-A, rel2, rel3, v-rel, xrel, REL proto-oncogene, NF-kB subunit, RELA proto-oncogene, NF-kB subunit, v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel avian reticuloendotheliosis viral oncogene homolog, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog, REL, RELA, Rela, Rel, rel, rel.L
    Background
    NFkB (Nuclear Factor NF-kappa-B) is a pleiotropic transcription factor that plays a role in many biological processes, including inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. It is found as a homo- or heterodimeric complex containing the Rel-like domain containing proteins NFkB p65 (RELA/p65), RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The heterodimeric NFkB p65/p50 complex is the most abundant one. The dimers bind to kappa-B sites at their target genes, with the affinity of the interaction dependent on the subunit composition of the dimer. Furthermore, different dimers act as transcriptional activators or repressors, with the NFkB p65/p50 and p65-c-Rel complexes acting as activators. NFkB activity is controlled by several different mechanisms, including post-translational modifications, subcellular localisation and interactions with other coactivators or corepressors. NFkB complexes are held in the cytoplasm in an inactive state by interaction with members of the NFkB inhibitor (IkB) family. Typically, phosphorylation of IkB by IkB kinases (IKKs) in response to different activators leads to degradation of the inhibitor, allowing NFkB to translocate into the nucleus. The inhibitory effect of IkBs is primarily exerted through their interaction with NfKB p65. NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
    Gene ID
    5970
    UniProt
    Q04206
    Pathways
    NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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