Western blot: 1/1000-1/2000 (use 0.15 M PBS with 0.1 % BSA and 0.1 % Na-Azide (ECL: 5 %milk powder in TBST) as dilution buffer). Recommended Positive Control: protein extracts from all vertebrate tissues and cells. Please note: This antibody is not recommended for Immunofluorescence and Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
P97 ATPase is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p.Synonyms: 15S Mg(2+)-ATPase p97 subunit, Transitional endoplasmic reticulum ATPase, Valosin-containing protein