PAK4 antibody (N-Term)

Catalog No. ABIN127123

Product Details anti-PAK4 Antibody

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody recognizes 72 kDa Pak4. It does not cross-react with other Paks.

Purification: Antigen affinity purified

Immunogen: Synthetic peptide corresponding to the N-terminus of human Pak4

Application Details

Application Notes: Western Blot: 1 μg/mL. Immunoprecipitation: 20 μg/mL. Immunohistochemistry: 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for PAK4

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Alternative Name: PAK4 (PAK4 Products)

Synonyms: 5730488L07Rik antibody, AW555722 antibody, mKIAA1142 antibody, im:5916170 antibody, wu:fd12b01 antibody, zgc:92014 antibody, p21 (RAC1) activated kinase 4 antibody, p21 protein (Cdc42/Rac)-activated kinase 4 antibody, PAK4 antibody, Pak4 antibody, pak4 antibody

Background: The PAK (p21-activated kinase) family of serine/threonine kinases plays an important role in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, etc. Several mechanisms that induce PAK activation have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain at the N-terminal region of PAK causes autophosphorylation and conformational change of PAK. More recently identified members PAK4, PAK5 and PAK6 have lower sequence similarity with PAK1-3 in the regulatory N-terminal region. It has been demonstrated that phosphorylation of serine 474 of PAK4 may play a pivotal role in the activity and function of PAK4 kinase.Synonyms: KIAA1142, PAK 4, PAK-4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4

Gene ID: 10298

NCBI Accession: NP_001014831

UniProt: O96013

Pathways: RTK Signaling