BRCA2 antibody (N-Term)

Catalog No. ABIN127309

Product Details anti-BRCA2 Antibody

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects BRCA2.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide mapping to the N-terminus of Human BRCA2.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. HeLa cell lysate can be used as a Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS pH 7.2, containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: BRCA2 / FANCD1 (BRCA2 Products)

Synonyms: BRCC2 antibody, BROVCA2 antibody, FACD antibody, FAD antibody, FAD1 antibody, FANCB antibody, FANCD antibody, FANCD1 antibody, GLM3 antibody, PNCA2 antibody, 30169 antibody, BcDNA:SD25109 antibody, CG13583 antibody, CG13584 antibody, CG30169 antibody, Dmel\\CG30169 antibody, brca2 antibody, dmbrca2 antibody, fancd1 antibody, BRCA2 antibody, brcc2 antibody, brovca2 antibody, facd antibody, fad antibody, fad1 antibody, fancb antibody, AI256696 antibody, AW045498 antibody, Fancd1 antibody, RAB163 antibody, BRCA2, DNA repair associated antibody, Breast cancer 2, early onset homolog antibody, breast cancer 2, early onset antibody, BRCA2 antibody, Brca2 antibody, brca2 antibody, LOC584780 antibody

Background: BRCA 1 and 2 are breast cancer susceptibility proteins that plays a role in DNA damage, repair, cell cycle progression, transcription and apoptosis, etc. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. BRCA2 has been shown to be required for localization of Rad51 to sites of DSBs (double stranded breaks) in DNA. Cells lacking BRCA1 and BRCA2 cannot repair DSBs through the Rad51-dependent process.Synonyms: Breast cancer type 2 susceptibility protein, FACD, Fanconi anemia group D1 protein

Gene ID: 675

NCBI Accession: NP_000050

UniProt: P51587

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location