BUB1 antibody (AA 281-419)

Catalog No. ABIN129514

Product Details anti-BUB1 Antibody

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Binding Specificity: AA 281-419

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 281-419 of human BUB1 protein.
Immunogentype:Recombinant

Clone: 14H5

Isotype: IgG

Application Details

Application Notes: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 160 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control.  For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8 OR 100% methanol at -20° C.  Permeabilize fixed cells with 0.5% Triton X-100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: BUB1 (BUB1 Products)

Synonyms: bub1a antibody, bub1l antibody, hbub1 antibody, xbub1 antibody, BUB1A antibody, BUB1L antibody, hBUB1 antibody, AL022991 antibody, Bub1a antibody, C80208 antibody, D2Xrf87 antibody, BUB1 mitotic checkpoint serine/threonine kinase antibody, BUB1, mitotic checkpoint serine/threonine kinase antibody, BUB1 antibody, bub1 antibody, Bub1 antibody

Background: BUB1 (also called Mitotic checkpoint serine/threonine-protein kinase BUB1and BUB1A) is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating BUB3, a member of the miotic checkpoint complex, and activating the spindle checkpoint.  Mutations in this gene have been associated with aneuploidy and several forms of cancer.   BUB1 is autophosphorylated when cells enter mitosis.  This protein is localized to the nucleus in interphase cells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. BUB1 is highly expressed in testis and thymus, less in colon, spleen, lung and small intestine.  Expression is associated with cells/tissues with a high mitotic index.
Synonyms: Budding uninhibited by benzimidazoles 1 homolog antibody, hBUB1 antibody, Mitotic checkpoint serine/threonine protein kinase BUB1 antibody

Gene ID: 699

NCBI Accession: NP_004327

UniProt: Q53QE4