NEDD9 antibody (AA 82-398)

Catalog No. ABIN129519

Product Details anti-NEDD9 Antibody

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Binding Specificity: AA 82-398

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEDD9 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Anti-HEF1 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 82-398 of human HEF1 protein.

Clone: 14A11

Isotype: IgG

Application Details

Application Notes: This monoclonal antibody has been tested for use in western blotting, immunoprecipitation and immunofluorescence.  This clone recognizes HEF1 under non-denaturing conditions.  Specific conditions for reactivity should be optimized by the end user.  Expect bands approximately 115 and 105 in size corresponding to isoforms of HEF1 protein by western blotting in the appropriate cell lysate or extract.  This antibody does not recognize p130Cas.  Sin1 has not been tested.  IF was performed using 4% PFA fixed cells.  This monoclonal mostly detects HEF1 localized at the focal adhesion sites.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-NEDD9 antibody (ABIN129519)

Pugacheva, Golemis: "The focal adhesion scaffolding protein HEF1 regulates activation of the Aurora-A and Nek2 kinases at the centrosome." in: Nature cell biology, Vol. 7, Issue 10, pp. 937-46, (2005) (PubMed).

Target Details for NEDD9

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Alternative Name: HEF1 (NEDD9 Products)

Synonyms: NEDD9 antibody, CAS-L antibody, CAS2 antibody, CASL antibody, CASS2 antibody, HEF1 antibody, Cas-L antibody, CasL antibody, E230025G09Rik antibody, neural precursor cell expressed, developmentally down-regulated 9 antibody, neural precursor cell expressed, developmentally down-regulated gene 9 antibody, NEDD9 antibody, Nedd9 antibody

Background: HEF1, also known as Enhancer of filamentation 1, CRK-associated substrate-related protein, CAS-L, CasL, p105 and Neural precursor cell expressed developmentally down-regulated 9 is the product of the NEDD9 (CASGL) gene.  HEF1 functions as a docking protein that plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. HEF1 may also function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation.  HEF1 may also play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells.  Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.  HEF1 forms a homodimer and can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas.   HEF1 also forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase and also interacts with MICAL and TXNL4/DIM1.  This protein localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus.  HEF1 is widely expressed although higher levels are detected in kidney, lung, and placental tissue.  HEF1 is also detected in T-cells, B-cells and diverse cell lines.  HEF1 is activated upon induction of cell growth.  Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55.  Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle.  Isoform p55 arises from p105 as a result of cleavage at a caspase
Synonyms: Cas like docking antibody, CASL antibody, Crk associated substrate related protein antibody, dJ49G10.2 antibody, dJ761I2.1 antibody, Enhancer of filamentation 1 antibody

Gene ID: 4739, 5453680

UniProt: Q14511