alpha Adaptin antibody (AA 3-14)

Catalog No. ABIN129553

Product Details anti-alpha Adaptin Antibody

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Binding Specificity: AA 3-14

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha Adaptin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Chimpanzee, Orang-Utan, Mouse (Murine), Rat (Rattus), Chicken, Dog (Canine)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 3-14 of Human AP2A1 and AP2A2 proteins.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 104-107 kDa in size corresponding to AP2A1 or AP2A2 proteins by western blotting in the appropriate cell lysate or extract. Multiple isoforms of each subunit exist for these proteins. The immunogen sequence is found in both AP2A1 and AP2A2. This antibody recognizes a band of approximately 100 kDa in multiple human cell lines and specific reactivity can be blocked with the immunizing peptide.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.7 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for alpha Adaptin

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Alternative Name: AP2A (AP2A1 Products)

Background: The AP2A1 and AP2A2 represent the AP2 alpha proteins that are found in the AP2 complex in clathrin-coated vesicles. AP2A is also known as Alpha-adaptin A, Adaptor protein complex AP-2 alpha-1 subunit, Clathrin assembly protein complex 2 alpha-A large chain, 100 kDa coated vesicle protein A and Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit.  The AP2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.
Synonyms: AP-2 complex subunit alpha-1, Adapter-related protein complex 2 alpha-1 subunit, Adaptor protein complex AP-2 subunit alpha-1 Alpha1-adaptin, Plasma membrane adaptor HA2/AP2 Clathrin assembly protein complex 2 alpha-A large chain

Gene ID: 161, 27477041

NCBI Accession: NP_036437

UniProt: O95782

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation