WRNIP1 antibody (Internal Region, Isoform 1, Isoform 2)

Catalog No. ABIN129611

Product Details anti-WRNIP1 Antibody

Target: WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))

Binding Specificity: Internal Region, Isoform 1, Isoform 2

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WRNIP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Monkey

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 and WHIP2 with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in western blotting against HEK293 whole cell lysates.  The antibody is also functional by ELISA.  Dilutions for western blotting represent a starting point dilution and further optimization may be required.  The antibody detects a band of approximately 96.0 kDa (predicted molecular weight: 72.2 kDa). Specific band detection by western blot is blocked by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane.  Reactivity in other immunoassays is unknown.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for WRNIP1

Target: WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))

Alternative Name: WHIP (WRNIP1 Products)

Synonyms: WRNIP1 antibody, wrnip1 antibody, RP11-420G6.2 antibody, WHIP antibody, bA420G6.2 antibody, zgc:85976 antibody, 4833444L21Rik antibody, Wrnip antibody, Whip antibody, Werner helicase interacting protein 1 antibody, Werner helicase interacting protein 1 L homeolog antibody, ATPase WRNIP1 antibody, WRNIP1 antibody, wrnip1.L antibody, wrnip1 antibody, EDI_075920 antibody, CpipJ_CPIJ017857 antibody, PITG_07216 antibody, VDBG_02914 antibody, Wrnip1 antibody, PGTG_08805 antibody

Background: Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging.  Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process.  A second isoform exists (WHIP2).
Synonyms: ATPase WRNIP 1 antibody, ATPase WRNIP1 antibody, bA420G6.2 antibody, FLJ22526 antibody, Putative helicase RUVBL antibody, RP11 420G6.2 antibody, Werner helicasae interacting protein 1 antibody

Gene ID: 56897, 55661735

UniProt: Q96S55