SPRY4 antibody (AA 306-322)

Catalog No. ABIN129626

Product Details anti-SPRY4 Antibody

Target: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Binding Specificity: AA 306-322

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Chimpanzee

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 306-322 of Human Sprouty-4 protein.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract.  Some non-specific banding may be observed using this antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for SPRY4

Target: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Alternative Name: Sprouty-4 (SPRY4 Products)

Synonyms: SPRY4 antibody, LOC100218545 antibody, HH17 antibody, A030006O18Rik antibody, sprouty4 antibody, sprouty RTK signaling antagonist 4 antibody, sprouty (Drosophila) homolog 4 antibody, sprouty homolog 4 (Drosophila) antibody, Spry4 antibody, SPRY4 antibody, spry4 antibody

Background: Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.  SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.
Synonyms: Sprouty-4, SPRY4

Gene ID: 81848, 42741659

UniProt: P08183