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SMAD3 antibody (pSer423, pSer425)

SMAD3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN129675
  • Target See all SMAD3 Antibodies
    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
    Binding Specificity
    • 33
    • 28
    • 28
    • 28
    • 20
    • 14
    • 14
    • 11
    • 11
    • 9
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 417-425, pSer423, pSer425
    Reactivity
    • 256
    • 150
    • 138
    • 28
    • 10
    • 9
    • 8
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 247
    • 23
    Rabbit
    Clonality
    • 230
    • 40
    Polyclonal
    Conjugate
    • 137
    • 19
    • 14
    • 14
    • 12
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    This SMAD3 antibody is un-conjugated
    Application
    • 230
    • 116
    • 107
    • 74
    • 59
    • 55
    • 55
    • 53
    • 28
    • 13
    • 10
    • 8
    • 5
    • 4
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Cross-Reactivity
    Xenopus laevis, Xenopus tropicalis, Zebrafish (Brachydanio rerio), Rat (Rattus), Mouse (Murine), Pig (Porcine), Sheep (Ovine), Chicken
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein.
    Isotype
    IgG
  • Application Notes
    This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.  Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Hutchinson, Darling, Nicolaou, Gori, Squair, Cohen, Hill, Sapkota: "Salt-inducible kinases (SIKs) regulate TGFβ-mediated transcriptional and apoptotic responses." in: Cell death & disease, Vol. 11, Issue 1, pp. 49, (2020) (PubMed).

    Stappenbeck, Wang, Tang, Zhang, Parhami: "Inhibition of Non-Small Cell Lung Cancer Cells by Oxy210, an Oxysterol-Derivative that Antagonizes TGFβ and Hedgehog Signaling." in: Cells, Vol. 8, Issue 10, (2020) (PubMed).

    Feng, Tang, Huang, Sun, You, Xiao, Lv, Xu, Lan: "TGF-β Mediates Renal Fibrosis via the Smad3-Erbb4-IR Long Noncoding RNA Axis." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 26, Issue 1, pp. 148-161, (2019) (PubMed).

    Subramanian, Kanzaki, Galloway, Schilling: "Mechanical force regulates tendon extracellular matrix organization and tenocyte morphogenesis through TGFbeta signaling." in: eLife, Vol. 7, (2019) (PubMed).

    Gao, Kanasaki, Li, Kitada, Okazaki, Koya: "βklotho is essential for the anti-endothelial mesenchymal transition effects of N-acetyl-seryl-aspartyl-lysyl-proline." in: FEBS open bio, Vol. 9, Issue 5, pp. 1029-1038, (2019) (PubMed).

    Tang, Tang, Xu, Li, Deng, Zhang: "Generation of Smurf2 Conditional Knockout Mice." in: International journal of biological sciences, Vol. 14, Issue 5, pp. 542-548, (2018) (PubMed).

    Li, Chung, Li, Overstreet, Gagnon, Grouix, Leduc, Laurin, Zhang, Harris: "Fatty acid receptor modulator PBI-4050 inhibits kidney fibrosis and improves glycemic control." in: JCI insight, Vol. 3, Issue 10, (2018) (PubMed).

    Li, Shi, Srivastava, Kitada, Nagai, Nitta, Kohno, Kanasaki, Koya: "FGFR1 is critical for the anti-endothelial mesenchymal transition effect of N-acetyl-seryl-aspartyl-lysyl-proline via induction of the MAP4K4 pathway." in: Cell death & disease, Vol. 8, Issue 8, pp. e2965, (2018) (PubMed).

    Chung, Overstreet, Li, Wang, Niu, Wang, Fan, Sasaki, Jin, Khodo, Gewin, Zhang, Harris: "TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration." in: JCI insight, Vol. 3, Issue 21, (2018) (PubMed).

    Tang, Heller, Meng, Yu, Tang, Zhou, Zhang: "Transforming Growth Factor-β (TGF-β) Directly Activates the JAK1-STAT3 Axis to Induce Hepatic Fibrosis in Coordination with the SMAD Pathway." in: The Journal of biological chemistry, Vol. 292, Issue 10, pp. 4302-4312, (2017) (PubMed).

    Subathra, Korrapati, Howell, Arthur, Shayman, Schnellmann, Siskind: "Kidney glycosphingolipids are elevated early in diabetic nephropathy and mediate hypertrophy of mesangial cells." in: American journal of physiology. Renal physiology, Vol. 309, Issue 3, pp. F204-15, (2015) (PubMed).

    Luo, Xu, Chen, Warburton, Dong, Qian, Selman, Gauldie, Kolb, Shi: "A novel profibrotic mechanism mediated by TGFβ-stimulated collagen prolyl hydroxylase expression in fibrotic lung mesenchymal cells." in: The Journal of pathology, Vol. 236, Issue 3, pp. 384-94, (2015) (PubMed).

    Herhaus, Al-Salihi, Macartney, Weidlich, Sapkota: "OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3." in: Nature communications, Vol. 4, pp. 2519, (2014) (PubMed).

    Kawamura, Maeda, Imamura, Setoguchi, Yokouchi, Ishidou, Komiya: "SnoN suppresses maturation of chondrocytes by mediating signal cross-talk between transforming growth factor-β and bone morphogenetic protein pathways." in: The Journal of biological chemistry, Vol. 287, Issue 34, pp. 29101-13, (2012) (PubMed).

    Beckham, Tuttle, Tyler: "Reovirus activates transforming growth factor beta and bone morphogenetic protein signaling pathways in the central nervous system that contribute to neuronal survival following infection." in: Journal of virology, Vol. 83, Issue 10, pp. 5035-45, (2009) (PubMed).

    Coffman, Coluccio, Planchart, Robertson: "Oral-aboral axis specification in the sea urchin embryo III. Role of mitochondrial redox signaling via H2O2." in: Developmental biology, Vol. 330, Issue 1, pp. 123-30, (2009) (PubMed).

    Yamashita, Fatyol, Jin, Wang, Liu, Zhang: "TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta." in: Molecular cell, Vol. 31, Issue 6, pp. 918-24, (2008) (PubMed).

    Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, (2003) (PubMed).

  • Target
    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
    Alternative Name
    SMAD3 (SMAD3 Products)
    Synonyms
    HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, LDS1C antibody, LDS3 antibody, MADH3 antibody, madh3 antibody, madh3a antibody, smad3 antibody, wu:fa99e03 antibody, XSmad3 antibody, XenMLP antibody, Xmad3 antibody, madh3-A antibody, Madh3 antibody, AU022421 antibody, Smad 3 antibody, mad3 antibody, madh3b antibody, zgc:92234 antibody, SMAD family member 3 antibody, SMAD family member 3a antibody, SMAD family member 3 L homeolog antibody, SMAD family member 3b antibody, SMAD3 antibody, smad3a antibody, smad3.L antibody, Smad3 antibody, smad3b antibody
    Background
    This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase.   These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway.  Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
    Synonyms: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody
    Gene ID
    4088, 5174513
    UniProt
    P84022
    Pathways
    Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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