CD151 antibody (pSer30)

Catalog No. ABIN129680

Product Details anti-CD151 Antibody

Target: CD151

Binding Specificity: AA 26-35, pSer30

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD151 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to phosphorylated Mer2 protein by western blotting in the appropriate cell lysate or extract.  Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for Mer2 phosphorylated at the pS30 residue.  Preparation of extracts from cells 4hr after initiation of meiosis is suggested.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for CD151

Target: CD151

Alternative Name: Mer2 (CD151 Products)

Synonyms: CD151 antibody, MGC83668 antibody, gp27 antibody, mer2 antibody, raph antibody, sfa1 antibody, peta-3 antibody, tspan24 antibody, PETA-3 antibody, SFA-1 antibody, Tspan24 antibody, GP27 antibody, MER2 antibody, RAPH antibody, SFA1 antibody, TSPAN24 antibody, CD151 molecule antibody, CD151 molecule (Raph blood group) L homeolog antibody, CD151 molecule (Raph blood group) antibody, CD151 antigen antibody, CD151 antibody, cd151.L antibody, cd151 antibody, Cd151 antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression.  It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog.  This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases.   Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that S-phase CDK associates with the replication fork.
Synonyms: Meiotic recombination 2 protein REC107

Gene ID: 853478, 1170924

UniProt: P21651