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Myosin antibody (AA 12-27)

Reactivity: Human WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN129691
  • Target See all Myosin Antibodies
    Myosin
    Binding Specificity
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 12-27
    Reactivity
    • 33
    • 10
    • 8
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 29
    • 9
    Rabbit
    Clonality
    • 29
    • 9
    Polyclonal
    Conjugate
    • 23
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Myosin antibody is un-conjugated
    Application
    • 20
    • 20
    • 11
    • 9
    • 7
    • 6
    • 4
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Cross-Reactivity
    Mouse (Murine), Rat (Rattus)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 12-27 of human myosin light chain protein.
    Isotype
    IgG
    Top Product
    Discover our top product Myosin Primary Antibody
  • Application Notes
    This affinity-purified antibody was tested by ELISA and immunoblotting and was found to be reactive with both the unphosphorylated and mono-phosphorylated forms of the protein.   Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.71 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Deng, Bhaidani, Sutherland, MacDonald, Walsh et al.: "Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth ..." in: PLoS ONE, Vol. 14, Issue 12, pp. e0226406, (2020) (PubMed).

    Lahey, Ronaghan, Shang, Dion, Désilets, Leduc, MacNaughton: "Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180259, (2017) (PubMed).

    Logue, Cartagena-Rivera, Baird, Davidson, Chadwick, Waterman: "Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration." in: eLife, Vol. 4, pp. e08314, (2016) (PubMed).

  • Target
    Myosin
    Abstract
    Myosin Products
    Synonyms
    CG3201 antibody, DmMLC_C antibody, Dmel\\CG3201 antibody, MyoII antibody, mlc-c antibody, nmELC antibody, MYOSIN antibody, myosin 1 antibody, DFNA4 antibody, DFNA4A antibody, MHC16 antibody, MYH17 antibody, NMHC II-C antibody, NMHC-II-C antibody, PNMHH antibody, myosin antibody, MYH1 antibody, MYH2 antibody, MYH3 antibody, MYH4 antibody, MYH8 antibody, adult antibody, embryonic antibody, 2400004E04Rik antibody, II-C antibody, NHMCII antibody, DROMHC antibody, Dm nmII antibody, DmnmII antibody, Dronm-MII antibody, E(br) antibody, MHC antibody, Mhc-c antibody, Mhc-c[1] antibody, Myo antibody, Myo II antibody, NMM antibody, Zip antibody, anon-WO0140519.37 antibody, l(2)02957 antibody, nmMHC antibody, nmy-2 antibody, zpr antibody, DmelCG15792 antibody, CG15792 antibody, Myosin light chain cytoplasmic antibody, hypothetical protein antibody, myosin 1 antibody, myosin heavy chain 9 antibody, myosin heavy chain 14 antibody, myosin, heavy chain 1B, skeletal muscle antibody, myosin, heavy polypeptide 14 antibody, zipper antibody, Mlc-c antibody, T22C1.6 antibody, ATM1 antibody, MYH9 antibody, MYH14 antibody, Myh14 antibody, MYH1B antibody, zip antibody
    Background
    Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail.  The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons.  Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction.   Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.  The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.  This sequence differs from that of the sarcomeric/
    Synonyms: Myosin regulatory light polypeptide 9 Myosin regulatory light chain 9 Myosin regulatory light chain MRLC1 Myosin regulatory light chain 2, smooth muscle isoform Myosin RLC MLC-2C 20 kDa myosin light chain
    Gene ID
    98932, 38605043
    UniProt
    P24844
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