RFP antibody (Biotin)

Catalog No. ABIN129733

Product Details anti-RFP Antibody (Biotin)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Polyclonal anti-RFP is designed to detect RFP and its variants.

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence derived from the mushroom polyp coral Discosoma.
Immunogentype:Recombinant

Isotype: IgG

Application Details

Application Notes: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-RFP antibody (Biotin) (ABIN129733)

Drost, Houtman, Cseresnyés, Niesner, Rinnenthal, Miller, Prokop, Heppner: "The Amyloid-beta rich CNS environment alters myeloid cell functionality independent of their origin." in: Scientific reports, Vol. 10, Issue 1, pp. 7152, (2020) (PubMed).

Herndler-Brandstetter, Ishigame, Shinnakasu, Plajer, Stecher, Zhao, Lietzenmayer, Kroehling, Takumi, Kometani, Inoue, Kluger, Kaech, Kurosaki, Okada, Flavell: "KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity." in: Immunity, Vol. 48, Issue 4, pp. 716-729.e8, (2019) (PubMed).

Amano, Densmore, Lanske: "Conditional Deletion of Indian Hedgehog in Limb Mesenchyme Results in Complete Loss of Growth Plate Formation but Allows Mature Osteoblast Differentiation." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 30, Issue 12, pp. 2262-72, (2016) (PubMed).

Beyer, Kelly, Miquerol: "Inducible Cx40-Cre expression in the cardiac conduction system and arterial endothelial cells." in: Genesis (New York, N.Y. : 2000), (2010) (PubMed).

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP (RFP Products)

Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583