RFP antibody (Red Fluorescent Protein) (AA 234) (Biotin)

Details for Product anti-RFP Antibody No. ABIN129733, Supplier: Log in to see
Antigen
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Epitope
AA 234
28
6
3
2
1
Reactivity
Discosoma
81
50
37
9
7
1
1
Host
Rabbit
94
37
14
9
1
Clonality
Polyclonal
Conjugate
This RFP antibody is conjugated to Biotin
8
7
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
132
67
60
53
32
27
6
4
4
2
2
1
1
1
1
1
1
Options
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Immunogen The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence derived from the mushroom polyp coral Discosoma.
Immunogentype:Recombinant
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Pre-adsorbed
Alternative Name RFP (RFP Antibody Abstract)
Background Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Application Notes Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.1 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (Biotin) (ABIN129733) Anti-RFP Antibody - Western Blot Western blot of RFP recombinant protein detected wit...
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (Biotin) (ABIN129733) Western blot of Biotin conjugated Rabbit Anti-RFP Pre-adsorbed antibody. Lane 1: RFP....
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (Biotin) (ABIN129733) Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. ...
Product cited in: Beyer, Kelly, Miquerol: "Inducible Cx40-Cre expression in the cardiac conduction system and arterial endothelial cells." in: Genesis (New York, N.Y. : 2000), 2010 (PubMed).

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