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LY9 antibody (APC)

CD229 Reactivity: Human FACS Host: Mouse Monoclonal HLy9-25 APC
Catalog No. ABIN1302635
  • Target See all LY9 (CD229) Antibodies
    LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
    Reactivity
    • 41
    • 5
    • 3
    Human
    Host
    • 27
    • 15
    • 1
    Mouse
    Clonality
    • 28
    • 15
    Monoclonal
    Conjugate
    • 20
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LY9 antibody is conjugated to APC
    Application
    • 22
    • 14
    • 13
    • 13
    • 11
    • 10
    • 10
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
    Cross-Reactivity (Details)
    Human
    Purification
    Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    CD299-transfected 300.19 pre-B cell line
    Clone
    HLy9-25
    Isotype
    IgG1
    Top Product
    Discover our top product CD229 Primary Antibody
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Reconstitution
    No reconstitution is necessary.
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Romero, Zapater, Calvo, Kalko, de la Fuente, Tovar, Ockeloen, Pizcueta, Engel: "CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 7033-42, (2005) (PubMed).

    de la Fuente, Tovar, Villamor, Zapater, Pizcueta, Campo, Bosch, Engel: "Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9." in: Blood, Vol. 97, Issue 11, pp. 3513-20, (2001) (PubMed).

  • Target
    LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
    Alternative Name
    CD229 (CD229 Products)
    Synonyms
    LY9 antibody, CD229 antibody, SLAMF3 antibody, hly9 antibody, mLY9 antibody, AI893573 antibody, Lgp100 antibody, Ly-9 antibody, T100 antibody, lymphocyte antigen 9 antibody, LY9 antibody, Ly9 antibody
    Background
    Lymphocyte antigen 9,CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.,SLAMF3, LY9
    Gene ID
    4063
    UniProt
    Q9HBG7
    Pathways
    BCR Signaling
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