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KCNQ4 antibody

KCNQ4 Reactivity: Mammalian WB, IHC, ISt Host: Mouse Monoclonal N43-6 unconjugated
Catalog No. ABIN1304779
  • Target See all KCNQ4 Antibodies
    KCNQ4 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4))
    Reactivity
    • 40
    • 13
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mammalian
    Host
    • 24
    • 16
    • 1
    Mouse
    Clonality
    • 25
    • 16
    Monoclonal
    Conjugate
    • 21
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This KCNQ4 antibody is un-conjugated
    Application
    • 29
    • 18
    • 17
    • 15
    • 14
    • 13
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt)
    Characteristics
    K+ channels
    Purification
    Purified: Hybridoma in vivo ascites fluid purified by SAS (Saturated Ammonium Sulfate) precipitation followed by anion exchange chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.
    Clone
    N43-6
    Isotype
    IgG1
    Top Product
    Discover our top product KCNQ4 Primary Antibody
  • Restrictions
    For Research Use only
  • Validation #102037 (Western Blotting)
    'Independent Validation' Badge
    by
    Dipartimento di Medicina e Scienze della Salute, Università degli Studi del Molise, Campobasso
    No.
    #102037
    Date
    10/31/2017
    Antigen
    Kv7.4/KCNQ4
    Lot Number
    440-2HK-65b
    Method validated
    Western Blotting
    Positive Control

    CHO cells transfected with a plasmid containing the cDNA for the longest isoform of the human Kv7.4/KCNQ4 channel

    Negative Control

    Non-transfected CHO cells (no Kv7 channel expression)

    Notes

    Passed. ABIN1304779 specifically detects KCNQ4/Kv7.4 in lysates from CHO cells expressing human KCNQ4/Kv7.4.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN654696
    Secondary Antibody
    goat anti-mouse IgG (H+L) antibody (ImmunoReagents, GTXMU-003-DHRPX, lot 31-118-043013)
    Full Protocol
    • Grow CHO cells (Chinese Hamster Ovary cells) in DMEM medium (Microgem, L0106-500) supplemented with 10% fetal bovine serum (Microgem, S1810-500) and antibiotics (1X Penicillin-Streptomycin mix, Microgem, L0022-100), at 37°C and 5% CO2 in 60mm plates.
    • Transfect CHO cells with 5µg plasmid expressing either Kv7.1, Kv7.2, Kv7.3, Kv7.4, or Kv7.5 and 1µg of plasmid expressing EGFP as a transfection marker using Lipofectamine 2000 (Invitrogen, 11668019) following the manufacturer´s instructions.
    • Lyse cells in an appropriate volume of B-buffer, containing: 150mM NaCl, 10mM Tris-HCl pH7.4, 1mM EDTA, 1% SDS and 1x protease inhibitors (Roche, 11836145001, lot 11231400).
    • Determine total protein content of the lysates using a Bradford protein assay (AppliChem, A6932, lot 6J015116).
    • Denature 20µg of total protein for 10min at 100°C in 1/3 volume of 4x Laemmli SDS sample buffer and subsequently separate them on a denaturing 8% SDS-PAGE gel in Mini-PROTEAN Electrophoresis Cells (Bio-Rad) for 2-3h at 100 V.
    • Transfer proteins onto PVDF filter (Bio-Rad, 1620177, lot 28369A01) with transfer buffer (25mM Tris, 192mM glycine, 20% methanol) in a western blotting system at 4°C at 40mA ON.
    • Block the membrane with blocking buffer (5% milk dissolved in PBS + 1% Tween20, PBS-T) for 1h at RT.
    • Incubation with primary
      • monoclonal mouse anti-KCNQ4/Kv7.4 antibody (antibodies-online, ABIN1304779, lot 440-2HK-65b) diluted 1:1000 in blocking buffer ON at 4°C or
      • mouse anti-alpha-tubulin (Sigma, T8203, lot 024M4827V) diluted 1:5000 in blocking buffer ON at 4°C.
    • Wash membrane 3x for 10min with PBS-T.
    • Incubation with secondary goat anti-mouse IgG (H+L) antibody (ImmunoReagents, GTXMU-003-DHRPX, lot 31-118-043013) diluted 1:5000 in blocking buffer for 1h at RT.
    • Wash membrane 3x for 10min with PBS-T.
    • Treat PVDF filters with chemiluminescence solutions (ECL, Promega) for 5min at RT.
    • Images were captured, stored, and analyzed with the ImageLab, version 4.1 analysis software (Bio-Rad).
    Experimental Notes
  • Format
    Liquid
  • Testai, Barrese, Soldovieri, Ambrosino, Martelli, Vinciguerra, Miceli, Greenwood, Curtis, Breschi, Sisalli, Scorziello, Canduela, Grandes, Calderone, Taglialatela: "Expression and function of Kv7.4 channels in rat cardiac mitochondria: possible targets for cardioprotection." in: Cardiovascular research, Vol. 110, Issue 1, pp. 40-50, (2016) (PubMed).

  • Target
    KCNQ4 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4))
    Alternative Name
    Kv7.4/KCNQ4 (KCNQ4 Products)
    Synonyms
    KCNQ4 antibody, k(v)7.4 antibody, kv7.4 antibody, DFNA2 antibody, DFNA2A antibody, KV7.4 antibody, potassium voltage-gated channel subfamily Q member 4 antibody, potassium channel, voltage gated KQT-like subfamily Q, member 4 antibody, potassium voltage-gated channel, subfamily Q, member 4 antibody, KCNQ4 antibody, kcnq4 antibody, Kcnq4 antibody
    UniProt
    P56696
    Pathways
    Sensory Perception of Sound
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