Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CD4 antibody

CD4 Reactivity: Rat FACS Host: Mouse Monoclonal W3-25 unconjugated
Catalog No. ABIN133174
  • Target See all CD4 Antibodies
    CD4
    Reactivity
    • 355
    • 199
    • 112
    • 38
    • 24
    • 20
    • 19
    • 18
    • 15
    • 11
    • 10
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 327
    • 146
    • 108
    • 4
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 444
    • 138
    • 3
    Monoclonal
    Conjugate
    • 194
    • 65
    • 54
    • 46
    • 30
    • 12
    • 11
    • 10
    • 9
    • 9
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This CD4 antibody is un-conjugated
    Application
    • 469
    • 193
    • 99
    • 93
    • 81
    • 76
    • 71
    • 42
    • 39
    • 37
    • 33
    • 24
    • 10
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    Rat CD4
    Purification
    Purified from ascitic fluid via Protein G Chromatography
    Immunogen
    Rat thymocyte membrane . Fusion Partner: P3-NSI/1-Ag 4 . Donor: BALB/c spleen
    Clone
    W3-25
    Isotype
    IgG1
    Top Product
    Discover our top product CD4 Primary Antibody
  • Application Notes
    This clone is reported to work with frozen and paraffin sections (11) and functional testing (12).
    Restrictions
    For Research Use only
  • Concentration
    0.5 µg/10 cells
    Buffer
    Purified Ig buffered in PBS and 0.02% NaN (Purified from ascitic fluid via Protein G Chromatography).
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Saravanan, Sareen, Abu-El-Rub, Ashour, Sequiera, Ammar, Gopinath, Shamaa, Sayed, Moudgil, Vadivelu, Dhingra: "Graphene Oxide-Gold Nanosheets Containing Chitosan Scaffold Improves Ventricular Contractility and Function After Implantation into Infarcted Heart." in: Scientific reports, Vol. 8, Issue 1, pp. 15069, (2019) (PubMed).

  • Target
    CD4
    Alternative Name
    CD4 (CD4 Products)
    Synonyms
    cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
    Background
    Anti-rat T helper cell monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells and peritoneal macrophages. (1,2,3,10.) The antigen recognized by this antibody is a surface glycoprotein of Mr 48 000 - 52 000 and is the homologue of the human CD4 and the mouse L3/T4 antigen. ABIN133174labels the rat T helper subset, which mediates the helper activity for B and T cells, graft vs. host (GVH) reactivity and produces IL-2 in the mixed lymphocyte reaction (MLR). (2,4,6) Addition of ABIN133174to the MLR, inhibited proliferation and blocks the production of IL- 2. (4,5,6,9) T cells which mediate cytotoxicity and suppressor functions are not labelled. (Thus, cells labelled by this antibody are not labelled by MRC OX-8.) ABIN133174is invaluable for separating T cell subsets for functional studies and for labelling cells in tissue sections. It has been used in studying the role of T lymphocytes in graft rejections (7) and in studying the subsets of T cells in the rat which mediate graft vs. host disease. (8) This particular antibody is also one of three antibodies which labels T lymphocyte populations in the rat. These clones include W3/13 ABIN133174, which labels all T cells, as well as MRC OX-8 ABIN133174 and W3/25 ABIN133174 which label non-overlapping T cell subpopulations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (11) and functional testing (12).
    Pathways
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
You are here:
Support