CD4 antibody (Biotin)

Catalog No. ABIN133176

Product Details anti-CD4 Antibody (Biotin)

Target: CD4

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: Rat CD4

Immunogen: Rat thymocyte membrane . Fusion Partner: P3-NSI-1-Ag4 (NSI/1). . Donor: BALB/c spleen

Clone: W3-25

Isotype: IgG1

Application Details

Application Notes: This clone is reported to work with frozen and paraffin sections (11).

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/ml

Buffer: Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD4

Target: CD4

Alternative Name: CD4 (CD4 Products)

Synonyms: cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

Background: Anti-rat CD4 monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells and peritoneal macrophages. (1,2,3,10.) The antigen recognized by this antibody is a surface glycoprotein of Mr 48,000- 52,000 and is the homologue of the human CD4 and the mouse L3/T4 antigen. ABIN133176labels the rat T helper subset, which mediates the helper activity for B and T cells, graft vs. host (GVH) reactivity and produces IL-2 in the mixed lymphocyte reaction (MLR). (2,4,6,) Addition of ABIN133176to the MLR, inhibited proliferation and blocks the production of IL- 2. (4,5,6,9) T cells which mediate cytotoxicity and suppressor functions are not labelled . (Thus, cells labelled by this antibody are not labelled by MRC OX-8.) ABIN133176is invaluable for separating T cell subsets for functional studies and for labelling cells in tissue sections. It has been used in studing the role of T lymphocytes in graft rejections (7) and in studying the subsets of T cells in the rat which mediate graft vs. host disease.(8) This particular antibody is also one of three antibodies which labels T lymphocyte populations in the rat. These clones include W3/13 ABIN133176, which labels all T cells as well as MRC OX-8 ABIN133176 and W3/25 ABIN133176 which label non-overlapping T cell subpopulations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (11).

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events