CD8 alpha antibody

Catalog No. ABIN133184

Product Details anti-CD8 alpha Antibody

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Rat CD8a

Purification: Ion exchange column

Immunogen: High molecular weight rat thymocyte glycoproteins . Fusion Partner: P3-NSI-1-Ag4 (NS1/1) . Donor: BALB/c spleen

Clone: OX-8

Isotype: IgG1

Application Details

Application Notes: This clone is reported to work with frozen and paraffin sections (9).

Restrictions: For Research Use only

Handling

Concentration: 0.1 µg/10 cells

Storage: 4 °C

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a (CD8A Products)

Synonyms: cd8 antibody, LOC100136450 antibody, BB154331 antibody, Ly-2 antibody, Ly-35 antibody, Ly-B antibody, Lyt-2 antibody, zgc:136643 antibody, CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, RHACD8-4 antibody, CD8a molecule antibody, uncharacterized LOC100125537 antibody, CD8 alpha antibody, CD8 antigen, alpha chain antibody, T-cell surface glycoprotein CD8 alpha chain antibody, Cd8a antibody, LOC100125537 antibody, LOC100136450 antibody, CD8A antibody, cd8a antibody, LOC100126556 antibody

Background: Anti-rat T cytotoxic/suppressor cell monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine (1,2,3,4). The antigen recognized is a complex of surface glycoproteins of Mr 34, 39, and 76 kDa and is the rat homologue of the human CD8 and the mouse Ly 2.3 antigen (5,8). ABIN133184labels all peripheral T cells that are unlabelled by the ABIN133184(W3/25) monoclonal antibody. It labels a T cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor (2). ABIN133184and ABIN133184can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft versus host disease (7). This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in the rat, these being ABIN133184(W3/13) which labels all T cells, as well as ABIN133184(W3/25) and ABIN133184(MRC OX-8) which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (9).

Pathways: TCR Signaling