RT1.B antibody, RT1 class II, locus Bb antibody, RT1-Bb antibody
Background
Anti-rat RT1.B monoclonal antibody recognizes Ia from all rat strains tested. It labels a monomorphic determinant of the rat Ia antigen present on the majority of peripheral B lymphocytes, dendritic cells, some macrophages, and certain epithelial cells (2,3,4) but not peripheral T lymphocytes. (4) This antibody also cross reacts with murine Ia antigens (particularly with k and s haplotypes). Analysis of recombinant strains shows that the determinant maps to the I-A region of the mouse MHC (1,2) and correlates with mouse Ia specificity 17 or 18. Thus, the determinant recognized by ABIN133238is polymorphic in the mouse. This antibody may be useful for recognizing the rat Ia antigen and can be coupled to Sepharose 4B for purification of rat Ia antigen by antibody affinity chromatography (1).