RT1-BB antibody (PE)

Catalog No. ABIN133240

Product Details anti-RT1-BB Antibody (PE)

Target: RT1-BB (RT1 Class II, Locus Bb (RT1-BB))

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RT1-BB antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Purification: Purified from ascitic fluid via Protein G Chromatography.

Immunogen: rat thymocyte membrane glycoprotein . Fusion Partner: P3-NS1-1-AG4 (NS1/1) Specificity Rat RT1.B (Rat Ia-non-polymorphic) . Donor: BALB/c spleen

Clone: OX-6

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/ml

Buffer: R-PE conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography).

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for RT1-BB

Target: RT1-BB (RT1 Class II, Locus Bb (RT1-BB))

Alternative Name: RT1.B (RT1-BB Products)

Synonyms: RT1.B antibody, RT1 class II, locus Bb antibody, RT1-Bb antibody

Background: Anti-rat RT1.B monoclonal antibody recognizes Ia from all rat strains tested. It labels a monomorphic determinant of the rat Ia antigen present on the majority of peripheral B lymphocytes, dendritic cells, some macrophages, and certain epithelial cells (2,3,4) but not peripheral T lymphocytes. (4) This antibody also cross reacts with murine Ia antigens (particularly with k and s haplotypes). Analysis of recombinant strains shows that the determinant maps to the I-A region of the mouse MHC (1,2) and correlates with mouse Ia specificity 17 or 18. Thus, the determinant recognized by ABIN133240is polymorphic in the mouse. This antibody may be useful for recognizing the rat Ia antigen and can be coupled to Sepharose 4B for purification of rat Ia antigen by antibody affinity chromatography (1).

Pathways: Production of Molecular Mediator of Immune Response