Mouse anti-Rat Ig (Chain kappa) Antibody

Catalog No. ABIN133280

Product Details

Target: Ig

Binding Specificity: Chain kappa

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Application: Flow Cytometry (FACS)

Specificity: Rat Ig (Kappa Chain)

Characteristics: anti-rat Ig chain monoclonal antibody recognizes a determinant on rat kappa chains and binds preferentially to the Ig-1b allotype. This antibody provides a suitable marker for virtually all immunoglobin, regardless of class since 5 % or less of rat light chains are lambda. ABIN133280 detects surface immunoglobulin of rat peripheral blood B lymphocytes and has been used in indirect binding assays for detecting rat antibodies. Applications include flow cytometry for labelling rat B lymphocytes.

Purification: Purified from ascitic fluid via Protein G Chromatography

Immunogen: Pooled rat serum IgG . Fusion Partner: P3-NSI-1-Ag4 (NS1/1) . Donor: BALB/c spleen

Clone: OX-12

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS and 0.02 % NaN.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Storage: 4 °C/-20 °C

Target Details

Target: Ig

Abstract: Ig Products