COL2 antibody

Catalog No. ABIN135048

Product Details anti-COL2 Antibody

Target: COL2 (Collagen, Type II (COL2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This COL2 antibody is un-conjugated

Application: ELISA

Specificity: Reacts with conformational determinants on type II collagen Referenced species reactivities include - Human 4,5,10,12,14,18-20,22,24,27,29,31,33,34 Chicken 16,26 Mouse 6,7,15 Porcine 21 Rat 13,17,25 Rabbit 2,9,11,30,32 Bovine 2,3,19,23 Sheep 8,28

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Goat Anti-Type II Collagen-UNLB

Purification: Purification Method: Affinity chromatography on type II collagen covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA
• Other referenced applications include - IHC-PS , IHC-FS , ICC , EM , WB , IP
• Working Dilutions: ELISA BIOT conjugate 1:1,000 - 1:4,000 FLISA AF488 conjugate 1:200 - 1:400

Sample Volume: 0,5 mL

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: 0.2 mg of purified immunoglobulin in 0.5 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-COL2 antibody (ABIN135048)

Tan Timur, Caron, van den Akker, van der Windt, Visser, van Rhijn, Weinans, Welting, Emans, Jahr: "Increased TGF-β and BMP Levels and Improved Chondrocyte-Specific Marker Expression In Vitro under Cartilage-Specific Physiological Osmolarity." in: International journal of molecular sciences, Vol. 20, Issue 4, (2019) (PubMed).

Kuwahara, Serowoky, Vakhshori, Tripuraneni, Hegde, Lieberman, Crump, Mariani: "Sox9+ messenger cells orchestrate large-scale skeletal regeneration in the mammalian rib." in: eLife, Vol. 8, (2019) (PubMed).

Fujii, Hirota, Yasoda, Takizawa, Morozumi, Nakamura, Yotsumoto, Kondo, Yamashita, Sakane, Kanai, Ueda, Yamauchi, Yamanaka, Nakao, Kuwahara, Jindo, Furuya, Mashimo, Inagaki, Serikawa, Nakao: "Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death." in: PLoS ONE, Vol. 13, Issue 3, pp. e0194812, (2018) (PubMed).

Caron, Emans, Sanen, Surtel, Cremers, Ophelders, van Rhijn, Welting: "The Role of Prostaglandins and COX-Enzymes in Chondrogenic Differentiation of ATDC5 Progenitor Cells." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153162, (2016) (PubMed).

Nakao, Osawa, Yasoda, Yamanaka, Fujii, Kondo, Koyama, Kanamoto, Miura, Kuwahara, Akiyama, Bessho, Nakao: "The Local CNP/GC-B system in growth plate is responsible for physiological endochondral bone growth." in: Scientific reports, Vol. 5, pp. 10554, (2016) (PubMed).

van den Akker, Surtel, Cremers, Rodrigues-Pinto, Richardson, Hoyland, van Rhijn, Welting, Voncken: "Novel immortal human cell lines reveal subpopulations in the nucleus pulposus." in: Arthritis research & therapy, Vol. 16, Issue 3, pp. R135, (2014) (PubMed).

Mirmalek-Sani, Orlando, McQuilling, Pareta, Mack, Salvatori, Farney, Stratta, Atala, Opara, Soker: "Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering." in: Biomaterials, Vol. 34, Issue 22, pp. 5488-95, (2013) (PubMed).

Target Details for COL2

Target: COL2 (Collagen, Type II (COL2))

Alternative Name: Type II Collagen (COL2 Products)

Synonyms: PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, prothrombin antibody, CAP18 antibody, Cnlp antibody, Cramp antibody, FALL39 antibody, MCLP antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, cathelicidin antimicrobial peptide antibody, Cuticle collagen 2 antibody, collagen type II alpha 1 chain antibody, F2 antibody, Camp antibody, col-2 antibody, COL2A1 antibody