Collagen IV antibody (Biotin)

Catalog No. ABIN135053

Product Details anti-Collagen IV Antibody (Biotin)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is conjugated to Biotin

Application: ELISA

Specificity: Reacts with conformational determinants on type IV collagen Referenced species reactivities include - Human 1,12,14,27,29,31,34,36 Chicken 5,13,15 Porcine 11,22,25 Mouse 4,5,7,8,30,32 Chinchilla 17 Rabbit 5,6,14,20,23 Rat 2,3,10,26,27,33,35 Equine 18,28 Sheep 24 Bovine 5,24 Guinea Pig 14 Tree Shrew 21 Canine 9,19 Japanese Macaque 16

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Goat Anti-Type IV Collagen-BIOT

Purification: Purification Method: Affinity chromatography on type IV collagen covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA
• Other referenced applications include - IHC-PS , IHC-FS , ICC , EM , FC , WB , IP
• Working Dilutions: ELISA BIOT conjugate 1:1,000 - 1:4,000 FLISA AF488 conjugate 1:200 - 1:400

Sample Volume: 0,5 mL

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: 0.2 mg in 0.5 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-Collagen IV antibody (Biotin) (ABIN135053)

Weiner, Shah, Angelopoulos, Bartakova, Pulido, Murphy, Nudleman, Daneman, Goldberg: "Cholinergic neural activity directs retinal layer-specific angiogenesis and blood retinal barrier formation." in: Nature communications, Vol. 10, Issue 1, pp. 2477, (2019) (PubMed).

Ka, Hsieh, Lin, Yang, Wu, Sytwu, Chen: "Decoy receptor 3 inhibits renal mononuclear leukocyte infiltration and apoptosis and prevents progression of IgA nephropathy in mice." in: American journal of physiology. Renal physiology, Vol. 301, Issue 6, pp. F1218-30, (2011) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Type IV Collagen (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody