TNFRSF8 antibody

Catalog No. ABIN135193

Product Details anti-TNFRSF8 Antibody

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This TNFRSF8 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Mouse CD30, Mr 105-120 kDa

Characteristics: Hamster Anti-Mouse CD30-LE/AF

Endotoxin Level: Low endotoxin

Immunogen: Mouse CD30-mouse IgG1 fusion protein

Clone: MCD30-1

Isotype: IgG

Application Details

Application Notes:

• Applications: FC - Quality tested , Stim - Reported in literature , Costim - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 3 g/106 cells FITC and BIOT conjugates 3 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro functional studies

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of PBS

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: -20 °C

Storage Comment: Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

Target Details for TNFRSF8

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Alternative Name: CD30 (TNFRSF8 Products)

Synonyms: CD30 antibody, D1S166E antibody, Ki-1 antibody, Cd30 antibody, Ki antibody, TNFRSF8 antibody, TNF receptor superfamily member 8 antibody, tumor necrosis factor receptor superfamily, member 8 antibody, TNFRSF8 antibody, Tnfrsf8 antibody

Background: CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. In anti-CD3-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN-γ production. While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.