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LY6C + LY6G antibody (Biotin)

Reactivity: Mouse FACS Host: Rat Monoclonal RB6-8C5 Biotin
Catalog No. ABIN135405
  • Target
    LY6C + LY6G
    Reactivity
    Mouse
    Host
    • 35
    • 1
    Rat
    Clonality
    • 35
    • 1
    Monoclonal
    Conjugate
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 36
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Flow Cytometry (FACS)
    Specificity
    Mouse Ly-6G/Ly-6C, Mr 21-25 kDa
    Characteristics
    Rat Anti-Mouse Ly-6G/Ly-6C-BIOT
    Immunogen
    Unknown
    Clone
    RB6-8C5
    Isotype
    IgG2b
  • Application Notes
    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , CMCD - Reported in literature , Depletion - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells SPRD, APC, CY5, PE/CY5.5, PE/CY7, APC/CY7, and AF700 0.03 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Comment

    In vitro complement-dependent cytotoxicity assays, In vivo depletion of Ly-6G+ cells

    Sample Volume
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    0.5 mg in 1.0 mL of PBS/Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Target
    LY6C + LY6G
    Alternative Name
    Ly-6G/Ly-6C
    Background
    Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow. In the periphery, Ly-6G is expressed predominantly on neutrophils.
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