CD3 epsilon antibody (PE)

Catalog No. ABIN135467

Product Details anti-CD3 epsilon Antibody (PE)

Target: CD3 epsilon (CD3E)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: Porcine CD3, Mr 23 kDa

Characteristics: Mouse Anti-Porcine CD3e-PE

Immunogen: Purified CD3 molecules from porcine thymus

Clone: PPT3

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Sep - Reported in literature , Stim - Reported in literature , Cyto - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD3 epsilon antibody (PE) (ABIN135467)

Chen, Hou, Li, Xiong, Liu: "Cloning and characterization of porcine 4Ig-B7-H3: a potent inhibitor of porcine T-cell activation." in: PLoS ONE, Vol. 6, Issue 6, pp. e21341, (2011) (PubMed).

Hosoya, Villena, Shimazu, Tohno, Fujie, Chiba, Shimosato, Aso, Suda, Kawai, Saito, Alvarez, Ikegami, Itoh, Kitazawa: "Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells." in: Veterinary research, Vol. 42, pp. 111, (2011) (PubMed).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e (CD3E Products)

Synonyms: cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody

Background: Porcine CD3 is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. MAb PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway