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EGF antibody (AA 1-30)

Name: EGF antibody (AA 1-30)
SKU: ABIN1385262
Price: 384.62 USD
Availability: InStock

EGF Reactivity: Human ELISA, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded human kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with EGF Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, FITC conjugated-FITC)used at 1:200 dilution for 40 minutes at 37°C.

IF (p)

1 image

Catalog No. ABIN1385262

Datasheet as PDF

Target See all EGF Antibodies

EGF (Epidermal Growth Factor (EGF))
Binding Specificity
45

15

10

8

7

7

7

7

6

6

6

5

5

4

4

4

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-30
Reactivity
144

85

50

12

11

5

4

1

1

1
Human
Host
209

30

9

3

1

1
Rabbit
Compatible Secondaries
Clonality
214

38
Polyclonal
Conjugate
113

36

19

14

7

7

5

5

5

5

5

5

4

4

4

4

4

2

1

1

1

1

1
This EGF antibody is un-conjugated
Application
200

86

62

52

52

29

25

24

17

16

14

14

8

8

4

4

3

2

2

1

1
ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human

Purification

Purified by Protein A.

Immunogen

MAP conjugated synthetic peptide derived from human EGF

Isotype

IgG

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anti-Epidermal Growth Factor (EGF) antibody
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anti-Epidermal Growth Factor (EGF) antibody
EGF Reactivity: Human WB, DB Host: Rabbit Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) (AA 609-751) antibody
EGF Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) antibody
EGF Reactivity: Human WB, ELISA, ICC Host: Mouse Monoclonal 4-00E-11 unconjugated

anti-Epidermal Growth Factor (EGF) antibody
EGF Reactivity: Mouse WB, ELISA, Neut Host: Goat Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibody
EGF Reactivity: Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) (AA 977-1029) antibody
EGF Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) (AA 609-751) antibody
EGF Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal 2# unconjugated

Application Notes

ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IEM 1:20-200
IGS 1:20-200
GICA 1:20-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

EGF (Epidermal Growth Factor (EGF))

Alternative Name

Egf (EGF Products)

Synonyms

HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody

Background

Synonyms: URG, HOMG4, Pro-epidermal growth factor, EGF
Background: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Gene ID

1950

UniProt

P01133

Pathways

NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation