EGFLAM antibody (AA 111-210)

Catalog No. ABIN1385746

Product Details anti-EGFLAM Antibody

Target: EGFLAM (EGF-Like, Fibronectin Type III and Laminin G Domains (EGFLAM))

Binding Specificity: AA 111-210

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGFLAM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Predicted Reactivity: Human,Mouse,Rat,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Pikachurin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for EGFLAM

Target: EGFLAM (EGF-Like, Fibronectin Type III and Laminin G Domains (EGFLAM))

Alternative Name: EGFLAM/Pikachurin (EGFLAM Products)

Background:

Synonyms: fibronectin type-III and laminin G-like domain-containing protein, Agrin-like protein, AGRINL, AGRNL, EGF-like, EGF-like, fibronectin type III and laminin G domains, EGF-like, fibronectin type-III and laminin G-like domain-containing protein, EGFLA_HUMAN, EGFLAM, FLJ39155, Pikachurin.

Background: EGFLAM is a 1,017 amino acid secreted protein containing three EGF-like domains, two fibronectin type-III domains, and three laminin G-like domains. Colocalizing with bassoon, CtBP and dystroglycan in photoreceptor synaptic terminals, EGFLAM is involved in retinal photoreceptor ribbon synapse formation. EGFLAM may also promote matrix assembly and cell adhesion. Existing as five alternatively spliced isoforms, the gene encoding EGFLAM maps to human chromosome 5p13.2. Chromosome 5 makes up approximately 6 % of the human genome and contains 181 million base pairs, which encode 1,000 genes. Cockayne syndrome, Treacher Collins syndrome, acute myelogenous leukemias and myelodysplastic syndrome are associated with genes present on chromosome 5.

Pathways: Glycosaminoglycan Metabolic Process