POU4F2 antibody (AA 301-400)

Catalog No. ABIN1385914

Product Details anti-POU4F2 Antibody

Target: POU4F2 (POU Domain, Class 4, Transcription Factor 2 (POU4F2))

Binding Specificity: AA 301-400

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU4F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BRN3B/POU4F2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for POU4F2

Target: POU4F2 (POU Domain, Class 4, Transcription Factor 2 (POU4F2))

Alternative Name: BRN3B (POU4F2 Products)

Synonyms: BRN3.2 antibody, BRN3B antibody, Brn-3b antibody, Brn-3.2 antibody, Brn3b antibody, Pou4f-rs1 antibody, mBrn3-3R antibody, brn-3b antibody, brn3.2 antibody, POU4F2 antibody, Xbrn3b antibody, brn3b antibody, brn3b-A antibody, POU class 4 homeobox 2 antibody, POU domain, class 4, transcription factor 2 antibody, POU class 4 homeobox 2 L homeolog antibody, POU4F2 antibody, Pou4f2 antibody, pou4f2 antibody, po4f2 antibody, pou4f2.L antibody

Background:

Synonyms: BRN3B, class 4, transcription factor 2, Brain specic homeobox/POU domain protein 3B, Brain-3B, Brain-specic homeobox/POU domain protein 3B, Brn-3B, BRN3.2, Brn3b POU domain transcription factor, PO4F2_HUMAN, POU class 4 homeobox 2, POU domain, POU domain class 4 transcription factor 2, POU domain protein, POU4F2.

Background: The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. Brn-1 and Brn-2 are class III POU (Pit-Oct-Unc) domain proteins, whereas Brn-3 is a class IV POU domain protein. Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor. Interestingly, Brn-3a has two functional transactivating domains, one at the amino-terminus and one at the carboxy-terminus. Brn-2 is thought to be involved in smooth muscle cell development and differentiation.

Gene ID: 5458

UniProt: Q12837

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Sensory Perception of Sound