PCYT1A antibody (AA 201-300)

Catalog No. ABIN1386503

Product Details anti-PCYT1A Antibody

Target: PCYT1A (Phosphate Cytidylyltransferase 1, Choline, alpha (PCYT1A))

Binding Specificity: AA 201-300

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCYT1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PCYT1A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PCYT1A

Target: PCYT1A (Phosphate Cytidylyltransferase 1, Choline, alpha (PCYT1A))

Alternative Name: PCYT1A (PCYT1A Products)

Synonyms: CCTA antibody, CT antibody, CTA antibody, CTPCT antibody, PCYT1 antibody, CCT-alpha antibody, CTP antibody, ccta antibody, cta antibody, ctpct antibody, pcyt1 antibody, CTalpha antibody, Cctalpha antibody, Ctpct antibody, Cttalpha antibody, zgc:110012 antibody, zgc:110237 antibody, phosphate cytidylyltransferase 1, choline, alpha antibody, phosphate cytidylyltransferase 1, choline, alpha L homeolog antibody, phosphate cytidylyltransferase 1, choline, alpha isoform antibody, phosphate cytidylyltransferase 1, choline, alpha b antibody, phosphate cytidylyltransferase 1, choline, alpha a antibody, PCYT1A antibody, Pcyt1a antibody, pcyt1a.L antibody, pcyt1a antibody, pcyt1ab antibody, pcyt1aa antibody

Background:

Synonyms: CCT A, CCT alpha, CCT-alpha, Choline phosphate cytidylyltransferase A, Choline-phosphate cytidylyltransferase A, CT A, CT antibody CTP:phosphocholine cytidylyltransferase A, CTPCT, PCY1A_HUMAN, PCYT1, Pcyt1a, Phosphate cytidylyltransferase 1 choline alpha, Phosphate cytidylyltransferase 1 choline alpha isoform, Phosphorylcholine transferase A.

Background: Increase in fetal surfactant synthesis and lung maturity is caused by the glucocorticoidal induction of enzymes required for phosphatidylcholine synthesis towards the end of gestation (1). The regulation of gestational age-dependent induction of phosphatidylcholine synthesis by glucocorticoids is still unclear (1). The rate-controlling enzyme in the phosphatidylcholine biosynthetic pathway is CTP-phosphocholine cytidylyltransferase A (CCT A) (24). In cultured eukaryotic cells, this enzyme is essential for survival (3). The alpha isoform is located in the nucleus and is regulated by reversible phosphorylation and membrane association (3). There is significant identity between the alpha-helical membrane-binding domains of CCT A and soybean oleosin (2). Expressed CCT A has lipid-dependent cytidylyltransferase activity (5). The gene which encodes CCT A maps to human chromosome 3q (4).