STXBP5 antibody (AA 235-380)

Catalog No. ABIN1386695

Product Details anti-STXBP5 Antibody

Target: STXBP5 (Syntaxin Binding Protein 5 (Tomosyn) (STXBP5))

Binding Specificity: AA 235-380

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STXBP5 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Tomosyn/STXBP5

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for STXBP5

Target: STXBP5 (Syntaxin Binding Protein 5 (Tomosyn) (STXBP5))

Alternative Name: Tomosyn/STXBP5 (STXBP5 Products)

Synonyms: LGL3 antibody, LLGL3 antibody, Nbla04300 antibody, 0710001E20Rik antibody, 4930565N16Rik antibody, AW742610 antibody, mKIAA1006 antibody, syntaxin binding protein 5 antibody, syntaxin binding protein 5 (tomosyn) antibody, STXBP5 antibody, Stxbp5 antibody

Background:

Synonyms: FLJ30922, Lethal2 giant larvae protein homolog 3, LGL3, LLGL3, MGC141942, MGC141968, Nbla04300, STXBP5, Syntaxin binding protein 5, Syntaxin-binding protein 5, Tomosyn 1, STXB5_HUMAN.

Background: WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. Tomosyn, also known as STXBP5 (syntaxin binding protein 5), LLGL3 or LGL3, is a 1,151 amino acid protein that localizes to the cytoplasm, as well as to the cell junction, secretory vesicles and to the peripheral membrane and contains one v-SNARE coiled-coil homology domain and 14 WD repeats. Interacting with Syntaxin 1 and Syntaxin 1B, Tomosyn functions as a regulator of neurotransmitter release and calcium-dependent exocytosis. Additionally, Tomosyn inhibits membrane fusion and may play a role in the assembly of SNARE complexes between transport vesicles and the plasma membrane. Multiple isoforms of Tomosyn exist due to alternative splicing events.