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CD8B antibody (AA 51-150)

Name: CD8B antibody (AA 51-150)
SKU: ABIN1387300
Price: 384.62 USD
Availability: InStock

CD8B Reactivity: Human WB, ELISA, IHC (fro), IF (p), IF (cc), ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-CD8B Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD8B Polyclonal Antibody, Unconjugated (ABIN1387300) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at

Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8B Polyclonal Antibody, Unconjugated (ABIN1387300) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

3 images

Catalog No. ABIN1387300

Datasheet as PDF

Target See all CD8B Antibodies

CD8B (CD8b Molecule (CD8B))
Binding Specificity
15

14

11

8

8

7

6

4

3

2

2

2

1

1

1

1
AA 51-150
Reactivity
73

31

23

13

5

4

4

4

4

3

2

2

2

2

1

1

1

1
Human
Host
76

38

21
Rabbit
Compatible Secondaries
Clonality
77

57

1
Polyclonal
Conjugate
57

17

15

13

8

7

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8B antibody is un-conjugated
Application
77

71

43

22

15

13

12

12

8

7

4

3

3

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity

Human

Predicted Reactivity

Mouse,Rat,Pig,Horse,Chicken,Guinea Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CD8B

Isotype

IgG

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anti-CD8b Molecule (CD8B) antibody
CD8B Reactivity: Human, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

anti-CD8b Molecule (CD8B) antibody
CD8B Reactivity: Rat FACS, WB, IP, IHC (fro) Host: Mouse Monoclonal 341 unconjugated

anti-CD8b Molecule (CD8B) antibody (FITC)
CD8B Reactivity: Rat FACS Host: Mouse Monoclonal 341 FITC

anti-CD8b Molecule (CD8B) antibody (PE)
CD8B Reactivity: Rat FACS Host: Mouse Monoclonal 341 PE

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-CD8b Molecule (CD8B) antibody
CD8B Reactivity: Rat FACS, WB, IP, IHC (fro), Func Host: Mouse Monoclonal 341 unconjugated

anti-CD8b Molecule (CD8B) antibody (PE)
CD8B Reactivity: Mouse FACS Host: Rat Monoclonal H35-17-2 PE

anti-CD8b Molecule (CD8B) antibody (FITC)
CD8B Reactivity: Chicken FACS Host: Mouse Monoclonal EP42 FITC

anti-CD8b Molecule (CD8B) (AA 1-243) antibody
CD8B Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

CD8B (CD8b Molecule (CD8B))

Alternative Name

CD8B (CD8B Products)

Background

Synonyms: LY3, P37, LEU2, LYT3, CD8B1, T-cell surface glycoprotein CD8 beta chain, CD8b
Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.

Gene ID

926

UniProt

P10966

Pathways

TCR Signaling