SEMA3E antibody (AA 401-450)

Catalog No. ABIN1387869

Product Details anti-SEMA3E Antibody

Target: SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))

Binding Specificity: AA 401-450

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA3E antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SEMA3E/Semaphorin 3E

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SEMA3E

Target: SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))

Alternative Name: SEMA3E/Semaphorin 3E (SEMA3E Products)

Synonyms: M-SEMAH antibody, M-SemaK antibody, SEMAH antibody, coll-5 antibody, 6430702L12 antibody, AA408817 antibody, Semah antibody, mKIAA0331 antibody, semaphorin 3E antibody, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E antibody, SEMA3E antibody, Sema3e antibody

Background:

Synonyms: Coll 5, Coll5, KIAA0331, M sema H, M SEMAH, M SemaK, Msema H, MSEMAH, MSemaK, SEM3E_HUMAN, Sema domain immunoglobulin domain Ig, Sema domain immunoglobulin domain Ig short basic domain secreted 3E, Sema domain, immunoglobulin domain Ig, short basic domain, secreted, semaphorin 3E, SEMA3E, SEMAH, Semaphorin 3E, Semaphorin-3E, Semaphorin3E, Short basic domain secreted semaphorin 3E, SEM3E_HUMAN.

Background: Semaphorins are a family of cell surface and secreted proteins involved in neural development that are conserved from insects to humans. Members of this family are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular semaphorin? domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. SEMA3E is a secreted semaphorin with 775 amino acids. Mutations in the SEMA3E gene are associated with CHARGE syndrome, a disorder characterized by cranial nerve dysfunction, coloboma of the eye, choanal atresia, inner and external ear abnormalities, cardiac anomalies, genitourinary abnormalities, and growth retardation.