UBE2D3 antibody (AA 55-147)

Catalog No. ABIN1387890

Product Details anti-UBE2D3 Antibody

Target: UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))

Binding Specificity: AA 55-147

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2D3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Pig,Horse,Chicken,Rabbit,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2D3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for UBE2D3

Target: UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))

Alternative Name: UBE2D3 (UBE2D3 Products)

Synonyms: E2(17)KB3 antibody, UBC4/5 antibody, UBCH5C antibody, eff antibody, fb36f02 antibody, wu:fb36f02 antibody, zgc:56340 antibody, 1100001F19Rik antibody, 9430029A22Rik antibody, AA414951 antibody, UBE2D3P antibody, Xubc4 antibody, e2(17)kb3 antibody, ubc4/5 antibody, ubch5c antibody, ube2d3.1 antibody, ube2d2 antibody, xubc4 antibody, wu:fj13d01 antibody, zgc:73200 antibody, ubiquitin conjugating enzyme E2 D3 antibody, ubiquitin-conjugating enzyme E2D 3 antibody, ubiquitin conjugating enzyme E2 D3 L homeolog antibody, ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast), like antibody, UBE2D3 antibody, ube2d3 antibody, Ube2d3 antibody, LOC100223566 antibody, ube2d3.L antibody, ube2d2l antibody

Background:

Synonyms: E217KB3, MGC43926, MGC5416, UB2D3_HUMAN, UBC 4/5, UBC4/5, UBC4/5 homolog yeast, UBCH 5C, UBCH5C, Ube2d3, Ubiquitin carrier protein, Ubiquitin carrier protein D3, Ubiquitin conjugating enzyme E2 17 kDa 3, Ubiquitin conjugating enzyme E2 D3, Ubiquitin conjugating enzyme E2D 3 homologous to yeast UBC4/5, Ubiquitin conjugating enzyme E2D 3 UBC4/5 homolog yeast, Ubiquitin conjugating enzyme E2D 3, Ubiquitin protein ligase D3, Ubiquitin-conjugating enzyme E2 D3, Ubiquitin-conjugating enzyme E217KB 3, Ubiquitin-conjugating enzyme E2-17 kDa 3, Ubiquitin-protein ligase D3.

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction.

Gene ID: 7323

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades