EPS15 antibody (AA 161-260) (Biotin)

Catalog No. ABIN1390171

Product Details anti-EPS15 Antibody (Biotin)

Target: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Binding Specificity: AA 161-260

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPS15 antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EPS15

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for EPS15

Target: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Alternative Name: EPS15 (EPS15 Products)

Synonyms: AF-1P antibody, AF1P antibody, MLLT5 antibody, CG16932 antibody, Dmel\\CG16932 antibody, EH1 antibody, EPS-15 antibody, Eps15 antibody, anon-WO0118547.85 antibody, eps-15 antibody, eps15 antibody, i31 antibody, 2410112D09Rik antibody, RGD1307641 antibody, epidermal growth factor receptor pathway substrate 15 antibody, Epidermal growth factor receptor pathway substrate clone 15 antibody, hypothetical protein antibody, EPS15 antibody, Eps-15 antibody, eps15 antibody, Eps15 antibody, PGTG_06574 antibody

Background:

Synonyms: AF 1P, AF 1p protein, AF1P, ALL1 fused gene from chromosome 1, Epidermal growth factor receptor pathway substrate 15, Epidermal growth factor receptor substrate 15, EPS 15, MLLT 5, MLLT5, Protein Eps 15, Protein Eps15, EPS15_HUMAN.

Background: Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation. Miscues in RTK signaling pathways can result in cellular transformation and ultimately in cancer. Two novel EGF receptor substrates designated EGF-receptor pathway substrates 8 and 15, or Eps8 and Eps15, have been described. Eps8 and Eps15 are proteins, respectively that become tyrosine phosphorylated subsequent to EGF stimulation. Overexpression of Eps15 in NIH/3T3 cells causes cellular transformation, implying involvement in the regulation of cell proliferation. Eps15 is capable of binding the amino terminal portion of Crk via a conserved proline-rich domain, characteristic of all Crk binding proteins (5). Overexpression of Eps8 in both fibroblasts and hematopoietic cells results in an increased mitogenic response to EGF. Eps8 has been shown to associate with the EGF receptor despite its lack of a functional SH2 domain. Further characterization suggests the protein has both a PH domain and a SH3 domain, the functional significance of which are not yet known.

Pathways: EGFR Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, EGFR Downregulation