NGEF antibody (AA 451-550) (Biotin)

Catalog No. ABIN1391395

Product Details anti-NGEF Antibody (Biotin)

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Binding Specificity: AA 451-550

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGEF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EPHEXIN

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for NGEF

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Alternative Name: Ephexin-1/EPHEXIN (NGEF Products)

Synonyms: Ephexin-1 antibody, ARHGEF27 antibody, EPHEXIN antibody, Tims2 antibody, ephexin antibody, Besh3 antibody, ephexin-1 antibody, neuronal guanine nucleotide exchange factor antibody, NGEF antibody, Ngef antibody

Background:

Synonyms: EPHEXIN, ARHGEF27, Ephexin-1, Eph-interacting exchange protein, Neuronal guanine nucleotide exchange factor, NGEF

Background: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).

Gene ID: 25791

UniProt: Q8N5V2

Pathways: Neurotrophin Signaling Pathway