NSG1 antibody (AA 1-100) (Alexa Fluor 350)

Catalog No. ABIN1398003

Product Details anti-NSG1 Antibody (Alexa Fluor 350)

Target: NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))

Binding Specificity: AA 1-100

Reactivity: Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSG1 antibody is conjugated to Alexa Fluor 350

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Monkey, Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NEEP21

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NSG1

Target: NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))

Alternative Name: NEEP21 (D4S234E Products)

Synonyms: D4S234 antibody, D4S234E antibody, NEEP21 antibody, P21 antibody, Neep21 antibody, m234 antibody, p21 antibody, Bsmrb antibody, PEP1 antibody, neuronal vesicle trafficking associated 1 antibody, neuron specific gene family member 1 antibody, NSG1 antibody, Nsg1 antibody

Background:

Synonyms: neuron-enriched endosomal 21 kDa protein, NSG1_HUMAN.

Background: NEEP21, also known as Brain neuron cytoplasmic protein 1, NSG1 (neuron-specific proteins family member 1), P21 or D4S234E, is a single pass type II membrane protein belonging to the NSG family. It is highly expressed during neuronal maturation but its expression is downregulated in adult tissues. NEEP21 predominantly localizes to Rab 4-positive early endosomes in the somatodendritic neuronal compartment and is essential for proper receptor sorting and recycling in neurons. It associates with GRIP1 and GluR-2 and mediates the surface expression of GluR-2. When this interaction is interrupted, GluR-2 accumulates in early endosomes and leads to changes in evoked synaptic current properties. In addition, NEEP21 forms a complex with the SNARE protein, Syntaxin 13 (also known as Syntaxin 12), and participates in the recycling of transferrin receptors (TFRs) and NTR2 (neurotensin receptor 2).