CYTIP antibody (AA 1-100) (Cy3)

Catalog No. ABIN1406931

Product Details anti-CYTIP Antibody (Cy3)

Target: CYTIP (Cytohesin 1 Interacting Protein (CYTIP))

Binding Specificity: AA 1-100

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYTIP antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PSCDBP/CYTIP

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CYTIP

Target: CYTIP (Cytohesin 1 Interacting Protein (CYTIP))

Alternative Name: PSCDBP/CYTIP (CYTIP Products)

Background:

Synonyms: B31, CASP, Cbp HE, CYBR, CYTHIP, Cytip, CYTIP_HUMAN, Cytohesin binder and regulator, Cytohesin-associated scaffolding protein, Cytohesin-binding protein HE, Cytohesin-interacting protein, HE antibody Pleckstrin Homology Sec7 and Coiled-Coil Domains Binding Protein, Pleckstrin homology Sec7 and coiled-coil domains-binding protein.

Background: CYTIP is a cytoplasmic protein that is involved in lymphocytic cell adhesion. Expressed primarily in hematopoetic cells, CYTIP regulates the activity of cytohesin-1 (an integrin-activating protein involved in cell adhesion) by mediating its recruitment to the leukocyte membrane. Through its ability to bind cytohesin-1, CYTIP is able to sequester it to the cytoplasm, thereby preventing cytohesin-1 translocation to lymphocytes and interrupting the flow of information in the cell adhesion pathway. CYTIP can be recruited from the cytoplasm to the membrane by leukocyte integrins which interact with CYTIP through its PDZ domain. After membrane translocation, CYTIP can be re-located to the cytoplasm via exposure to a phorbol ester. Additionally, CYTIP associates with SNX27 (sorting nexin 27) and helps to coordinate trafficking and signaling complexes. Up-regulation of CYTIP is observed in maturing dendritic cells, suggesting a possible role in developmentally-controlled cell adhesion.