FBXW8 antibody (AA 181-280) (HRP)

Catalog No. ABIN1416712

Product Details anti-FBXW8 Antibody (HRP)

Target: FBXW8 (F-Box and WD Repeat Domain Containing 8 (FBXW8))

Binding Specificity: AA 181-280

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW8 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FBXW8/FBXO29

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FBXW8

Target: FBXW8 (F-Box and WD Repeat Domain Containing 8 (FBXW8))

Alternative Name: FBXW8 (FBXW8 Products)

Synonyms: FBW6 antibody, FBW8 antibody, FBX29 antibody, FBXO29 antibody, FBXW6 antibody, 4930438M06Rik antibody, Fbx29 antibody, F-box and WD repeat domain containing 8 antibody, ring finger protein, transmembrane 2 antibody, F-box and WD-40 domain protein 8 antibody, FBXW8 antibody, Fbxw8 antibody, RNFT2 antibody

Background:

Synonyms: F box/WD repeat containing protein 8; F box and WD40 domain containing protein 8; F-box only protein 29; F box only protein 29; FBW6; FBW8; FBX29; FBXO29; FBXW6; FBXW8_HUMAN.

Background: FBXO29, also designated F-box/WD repeat-containing protein 8 (FBXW8), is a 598 amino acid protein that contains one 40 amino acid F-box region, making it a member of the F-box family. FBXO29 also contains five WD repeats. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. F-box proteins are members of a large family that regulates cell cycle, immune response, signaling cascades and developmental programs by targeting proteins, such as cyclins, cyclin-dependent kinase inhibitors, IkB-å and -catenin, for degradation by the proteasome after ubiquitination. Functioning as a component of the SCF complex, FBXO29 is thought to recognize and bind to select phosphorylated proteins, thereby promoting their ubiquitination and subsequent degradation. FBXO29 exists as two isoforms as a result of alternative splicing events.

Gene ID: 26259