POLB antibody (AA 55-160) (Cy5.5)

Catalog No. ABIN1417423

This Rabbit Polyclonal antibody specifically detects POLB in WB, IF (cc) and IF (p). It exhibits reactivity toward Mouse.

Quick Overview for POLB antibody (AA 55-160) (Cy5.5) (ABIN1417423)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-POLB Antibody (Cy5.5)

Binding Specificity: AA 55-160

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA Polymerase beta

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: DNA Polymerase beta

Background:

Synonyms: DNA polymerase beta, POLB

Background: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.

Gene ID: 5423

UniProt: P06746

Pathways: DNA Damage Repair