CAMK2A antibody (Calcium/calmodulin-Dependent Protein Kinase II alpha)

Details for Product anti-CAMK2A Antibody No. ABIN151484
Antigen
Reactivity
Rat (Rattus)
148
97
94
5
4
3
2
1
1
1
1
1
1
1
Host
Mouse
147
34
7
Clonality (Clone)
Monoclonal ()
Conjugate
This CAMK2A antibody is un-conjugated
11
8
8
6
5
5
5
5
5
3
3
3
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
150
71
41
35
25
21
12
11
8
5
5
5
2
1
Options
Immunogen Detects approx. 50-60kDa protein, corresponding to the molecular mass of CaMKII alpha-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms.Partially purified rat CaMKII
Clone 6G9
Isotype IgG1
Specificity Detects approx 50-60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.
Purification Protein G purified
Antigen
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background Gene Symbol: CAMK2A
Molecular Weight Theoretical MW: 54 kDa
Gene ID 815
UniProt P11275
Pathways WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
Application Notes Western Blot 0.1 μg/mL, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500RadioImmunoassay 1:100-1:2000, Immunohistochemistry-Paraffin 1:10-1:500, Radioimmunoassay 1:100-1:20000.1 μg/mL was sufficient for detection of CamKII in 20 μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4) and 50 % Glycerol
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibody (ABIN151484) Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [ABIN15148...
Image no. 2 for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibody (ABIN151484) Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [ABIN15148...
Image no. 3 for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibody (ABIN151484) Western Blot: CaM Kinase II alpha Antibody (6G9) [ABIN151484] - analysis of Rat brain...
Image no. 4 for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibody (ABIN151484) Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [ABIN151484] - Tissue: colon...
Image no. 5 for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibody (ABIN151484) Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [ABIN15148...
Product cited in: Koene, van Grondelle, Proietti Onori, Wallaard, Kooijman, van Oort, Schreiber, Elgersma: "Effects of antiepileptic drugs in a new TSC/mTOR-dependent epilepsy mouse model." in: Annals of clinical and translational neurology, Vol. 6, Issue 7, pp. 1273-1291, 2019 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Schiapparelli, Shah, Ma, McClatchy, Sharma, Li, Yates, Goldberg, Cline: "The Retinal Ganglion Cell Transportome Identifies Proteins Transported to Axons and Presynaptic Compartments in the Visual System In Vivo." in: Cell reports, Vol. 28, Issue 7, pp. 1935-1947.e5, 2019 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Rat (Rattus)).

Liu, McClatchy, Schiapparelli, Shen, Yates, Cline: "Role of the visual experience-dependent nascent proteome in neuronal plasticity." in: eLife, Vol. 7, 2018 (PubMed).

Fang, Li, Huang, Zhang, Chen, Zhang, Ding, Shi, Lu, Yang: "Traumatic Stress Produces Distinct Activations of GABAergic and Glutamatergic Neurons in Amygdala." in: Frontiers in neuroscience, Vol. 12, pp. 387, 2018 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Rat (Rattus)).

Li, You, Zhang, Huang, Zou, Jie, Li, Liu, Lv, Cong, Hu, Gao, Li: "Satb2 Ablation Impairs Hippocampus-Based Long-Term Spatial Memory and Short-Term Working Memory and Immediate Early Genes (IEGs)-Mediated Hippocampal Synaptic Plasticity." in: Molecular neurobiology, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC), Western Blotting (WB) (Sample species: Mouse (Murine)).

Balu, Takagi, Puhl, Benneyworth, Coyle: "D-serine and serine racemase are localized to neurons in the adult mouse and human forebrain." in: Cellular and molecular neurobiology, Vol. 34, Issue 3, pp. 419-35, 2014 (PubMed).

Hamada, Ogawa, Yamasaki, Kiyama, Kassai, Watabe, Nakao, Aiba, Watanabe, Manabe: "The glutamate receptor GluN2 subunit regulates synaptic trafficking of AMPA receptors in the neonatal mouse brain." in: The European journal of neuroscience, Vol. 40, Issue 8, pp. 3136-46, 2014 (PubMed).

George, Baden, Gerwick, Murray: "Bidirectional influence of sodium channel activation on NMDA receptor-dependent cerebrocortical neuron structural plasticity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 48, pp. 19840-5, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Feng, Szabo, Dziak, Opas: "Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells." in: Stem cell reviews, Vol. 6, Issue 1, pp. 74-85, 2010 (PubMed).