CD74 antibody (HLA-DR-gamma) (AA 12-27)

Details for Product anti-CD74 Antibody No. ABIN151486
Antigen
Epitope
AA 12-27
42
20
16
15
6
5
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
452
97
31
22
Host
Mouse
383
100
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD74 antibody is un-conjugated
32
30
18
14
12
9
7
7
7
7
7
7
7
7
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
285
284
207
98
77
60
56
48
46
40
14
13
10
2
2
2
2
2
1
1
Options
Immunogen Peptide corresponding to residues 12-27 of CD74, human invariant chain (short form).
Clone PIN-1
Isotype IgG1
Specificity This detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.
No Cross-Reactivity Mouse (Murine)
Purification Protein G purified
Antigen
Alternative Name CD74 (CD74 Antibody Abstract)
Background Gene Symbol: CD74
Gene ID 972
UniProt P04233
Pathways Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
Application Notes Western Blot 1 μg/mL, Flow Cytometry 1 μg/million cells, Immunohistochemistry 5 μg/mL, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 5 μg/mL, CyTOF-readyIn Simple Western only 10-15 μL of the recommended dilution is used per data point. In WB this detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-HLA-DR-gamma (CD74) (AA 12-27) antibody (ABIN151486) Flow Cytometry: CD74 Antibody (PIN.1) [ABIN151486] - CD74 in human PBMCs (gated on al...
Image no. 2 for anti-HLA-DR-gamma (CD74) (AA 12-27) antibody (ABIN151486) Western Blot: CD74 Antibody (PIN.1) [ABIN151486] - Ramos whole cell protein was separ...
Image no. 3 for anti-HLA-DR-gamma (CD74) (AA 12-27) antibody (ABIN151486) anti-HLA-DR-gamma (CD74) (AA 12-27) antibody (Image 3)
Image no. 4 for anti-HLA-DR-gamma (CD74) (AA 12-27) antibody (ABIN151486) Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [ABIN151486] - IHC analysis of f...
Image no. 5 for anti-HLA-DR-gamma (CD74) (AA 12-27) antibody (ABIN151486) Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [ABIN151486] - IHC analysis of f...
Image no. 6 for anti-HLA-DR-gamma (CD74) (AA 12-27) antibody (ABIN151486) Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [ABIN151486] - IHC analysis of f...
Product cited in: Tanese, Hashimoto, Berkova, Wang, Samaniego, Lee, Ekmekcioglu, Grimm: "Cell Surface CD74-MIF Interactions Drive Melanoma Survival in Response to Interferon-γ." in: The Journal of investigative dermatology, Vol. 135, Issue 11, pp. 2775-84, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Katsel, Tan, Haroutunian: "Gain in brain immunity in the oldest-old differentiates cognitively normal from demented individuals." in: PLoS ONE, Vol. 4, Issue 10, pp. e7642, 2009 (PubMed).

Lamb, Cresswell: "Assembly and transport properties of invariant chain trimers and HLA-DR-invariant chain complexes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3478-82, 1992 (PubMed).

Roche, Marks, Cresswell: "Formation of a nine-subunit complex by HLA class II glycoproteins and the invariant chain." in: Nature, Vol. 354, Issue 6352, pp. 392-4, 1992 (PubMed).