Thrombospondin 1 antibody (THBS1)

Details for Product anti-THBS1 Antibody No. ABIN151527
Antigen
  • THBS
  • THBS-1
  • TSP
  • TSP-1
  • TSP1
  • Thbs-1
  • tbsp1
  • thrombospondin-1
  • thbs
  • thbs-1
  • tsp
  • tsp-1
  • tsp1
  • Tsp1
  • THBS1
  • Tsp-1
  • wu:fc48c03
  • si:dkey-11e23.1
  • tsp2
  • thrombospondin 1
  • thrombospondin 1 S homeolog
  • thrombospondin 1b
  • thrombospondin 2 L homeolog
  • THBS1
  • Thbs1
  • thbs1.S
  • thbs1b
  • thbs1
  • thbs2.L
Alternatives
anti-Mammalian Thrombospondin 1 antibody for Immunofluorescence
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
160
64
53
16
9
9
8
8
2
2
2
1
Host
Mouse
140
39
32
Clonality (Clone)
Monoclonal ()
Conjugate
This Thrombospondin 1 antibody is un-conjugated
29
12
9
3
3
2
2
2
2
2
2
2
1
Application
Blocking Peptide (BP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Western Blotting (WB)
158
97
65
29
25
23
17
14
10
4
4
3
3
2
2
2
2
2
2
2
1
Options
Immunogen BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
Clone A6-1
Isotype IgG1
Specificity This is specific to 450 kD protein, a non-reduced form, and 170 to 180 kD protein, a reduced form, of Thrombospondin.
Purification Protein A or G purified
Plasmids, Primers & others Plasmids, Primers & others Thrombospondin 1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Thrombospondin-1 (THBS1 Antibody Abstract)
Background Gene Symbol: THBS1
Gene ID 7057
UniProt P07996
Pathways Autophagy
Application Notes Western Blot 1:1000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:25-1:50, Blocking/NeutralizingThis antibody works in Immunocytochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot. This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Use in Blocking/Neutralizing reported in scientific literature (PMID: 19509293)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS
Buffer contains: Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Immunohistochemistry (IHC) image for anti-Thrombospondin 1 (THBS1) antibody (ABIN151527) Formalin-fixed, paraffin-embedded human placenta stained with Thrombospondin using pe...
Western Blotting (WB) image for anti-Thrombospondin 1 (THBS1) antibody (ABIN151527) anti-Thrombospondin 1 (THBS1) antibody (Image 2)
Western Blotting (WB) image for anti-Thrombospondin 1 (THBS1) antibody (ABIN151527) anti-Thrombospondin 1 (THBS1) antibody (Image 3)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Thrombospondin 1 (THBS1) antibody (ABIN151527) Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [ABIN151527] - Human ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Thrombospondin 1 (THBS1) antibody (ABIN151527) Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [ABIN151527] - Analys...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Thrombospondin 1 (THBS1) antibody (ABIN151527) Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) - Staining in human ...
Product cited in: Chen, Yang, Tian, Hong, Du, Li, Li, Wang, Yu, Wei: "Dental Follicle Stem Cells Ameliorate Lipopolysaccharide-Induced Inflammation by Secreting TGF-β3 and TSP-1 to Elicit Macrophage M2 Polarization." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 51, Issue 5, pp. 2290-2308, 2019 (PubMed). Method employed by authors: Neutralization (Neut) (Sample species: Rat (Rattus)).

Mouton, Ma, Rivera Gonzalez, Daseke, Flynn, Freeman, Garrett, DeLeon-Pennell, Lindsey: "Fibroblast polarization over the myocardial infarction time continuum shifts roles from inflammation to angiogenesis." in: Basic research in cardiology, Vol. 114, Issue 2, pp. 6, 2019 (PubMed). Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Chen, Ning, Xu, Jiang, Xing, Jia, Chen, Tang, Huang: "The miR-181d-regulated metalloproteinase Adamts1 enzymatically impairs adipogenesis via ECM remodeling." in: Cell death and differentiation, Vol. 23, Issue 11, pp. 1778-1791, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Cupi, Sarra, De Nitto, Franzè, Marafini, Monteleone, Del Vecchio Blanco, Paoluzi, Di Fusco, Gentileschi, Ortenzi, Colantoni, Pallone, Monteleone: "Defective expression of scavenger receptors in celiac disease mucosa." in: PLoS ONE, Vol. 9, Issue 6, pp. e100980, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Jayachandran, Anaka, Prithviraj, Hudson, McKeown, Lo, Vella, Goding, Cebon, Behren: "Thrombospondin 1 promotes an aggressive phenotype through epithelial-to-mesenchymal transition in human melanoma." in: Oncotarget, Vol. 5, Issue 14, pp. 5782-97, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Stawski, Haines, Fine, Rudnicka, Trojanowska: "MMP-12 deficiency attenuates angiotensin II-induced vascular injury, M2 macrophage accumulation, and skin and heart fibrosis." in: PLoS ONE, Vol. 9, Issue 10, pp. e109763, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Michaud-Levesque, Richard: "Thrombospondin-1 is a transcriptional repression target of PRMT6." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21338-46, 2009 (PubMed). (Sample species: Human). Further details: Blocking Peptide,Neutralization

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