p65 antibody (Nuclear Factor-KB P65) (C-Term)

Details for Product anti-NFkBP65 Antibody No. ABIN151596
Target
Binding Specificity
C-Term
39
38
29
28
28
24
23
21
19
18
17
16
16
15
14
14
13
13
12
12
10
9
9
9
8
8
8
6
6
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse, Rat
625
445
372
58
51
38
19
18
15
10
9
7
5
3
2
1
1
1
Host
Rabbit
581
54
6
1
1
Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
40
23
23
20
19
19
19
19
18
10
9
8
8
8
8
8
8
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
440
231
217
114
106
91
82
50
32
20
15
12
12
11
11
8
4
4
3
1
Options
Immunogen Synthetic peptide corresponding to the C-terminus of human NFkB p65.
Specificity Reacts with NFkB p65
Purification IgG purified
Target
Alternative Name RelA/NFkB p65 (NFkBP65 Antibody Abstract)
Background Gene Symbol: RELA
Molecular Weight Theoretical MW: 60.219 kDa
Gene ID 5970
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
Application Notes Western Blot 1:400-1:1000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500In Western Blot this antibody detects a band of approximately 64 kDa (predicted molecular weight: 69 kDa). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27119753).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS with 0.2 gelatin
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Images
Image no. 1 for anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody (ABIN151596) Immunohistochemistry: NF-kB p65 Antibody [ABIN151596] - Immunoperoxidase staining of ...
Image no. 2 for anti-Nuclear Factor-KB P65 (NFkBP65) (C-Term) antibody (ABIN151596) Western Blot: NF-kB p65 Antibody [ABIN151596] - Western blot analysis of NFkB p65 exp...
Product cited in: Sheng, Mao, Wang, Yang, Zhang, Han: "Dehydrocostus Lactone Enhances Chemotherapeutic Potential of Doxorubicin in Lung Cancer by Inducing Cell Death and Limiting Metastasis." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 7850-7861, 2019 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kim, Joe, Chen, Park, Kim, Chung: "Carbon monoxide attenuates amyloidogenesis via down-regulation of NF-κB-mediated BACE1 gene expression." in: Aging cell, Vol. 18, Issue 1, pp. e12864, 2019 (PubMed). Method employed by authors: Chromatin Immunoprecipitation (ChIP) (Sample species: Mouse (Murine)).

Li, Li, Zhuang, Li: "Inhibition of microRNA-34a protects against propofol anesthesia-induced neurotoxicity and cognitive dysfunction via the MAPK/ERK signaling pathway." in: Neuroscience letters, Vol. 675, pp. 152-159, 2018 (PubMed).

Zaky, Bouali-Benazzouz, Favereaux, Tell, Landry: "APE1/Ref-1 redox function contributes to inflammatory pain sensitization." in: Experimental neurology, Vol. 307, pp. 1-11, 2018 (PubMed).

Aggarwal, Makarem, Shah, Emin, Wei, St-Onge, Jelic: "Effects of Inadequate Sleep on Blood Pressure and Endothelial Inflammation in Women: Findings From the American Heart Association Go Red for Women Strategically Focused Research Network." in: Journal of the American Heart Association, Vol. 7, Issue 12, 2018 (PubMed).

Li, Luco, Ochietti, Fadhil, Camirand, Reinhardt, St-Arnaud, Muller, Kremer: "Tumoral Vitamin D Synthesis by CYP27B1 1-α-Hydroxylase Delays Mammary Tumor Progression in the PyMT-MMTV Mouse Model and Its Action Involves NF-κB Modulation." in: Endocrinology, Vol. 157, Issue 6, pp. 2204-16, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Ribes, Casemayou, El Hachem, Laurent, Guilbeau-Frugier, Vergez, Tavitian, Schanstra, Chauveau, Bascands, Ysebaert, Faguer: "Asymptomatic circulating T-cell clone cause renal polymorphic inflammatory fibrosis." in: Clinical and experimental nephrology, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Liu, Guo, Wu, Wang, Liu: "Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway." in: Molecular medicine reports, Vol. 15, Issue 1, pp. 169-179, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Sahbaz, Aynioglu, Isik, Gulle, Akpolat Ferah, Cicekler Sahbaz: "Cholecalciferol (vitamin D3) prevents postoperative adhesion formation by inactivating the nuclear factor kappa B pathway: a randomized experimental study." in: The Journal of surgical research, Vol. 198, Issue 1, pp. 252-9, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Zhu, Moore, Morii, Howard, Culver, Arrendale, Reddy, Evers, Zhang, Sica, Chen, Sun, Fu, Khuri, Shin, Snyder, Shoji: "Synthetic curcumin analog UBS109 inhibits the growth of head and neck squamous cell carcinoma xenografts." in: Current cancer drug targets, Vol. 14, Issue 4, pp. 380-93, 2014 (PubMed).

Zaky, Mohammad, Moftah, Kandeel, Bassiouny: "Apurinic/apyrimidinic endonuclease 1 is a key modulator of aluminum-induced neuroinflammation." in: BMC neuroscience, Vol. 14, Issue 1, pp. 26, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Zhu, Moore, Lin, Morii, Mancini, Howard, Culver, Arrendale, Reddy, Evers, Zhang, Sica, Chen, Sun, Fu, Khuri, Shin, Snyder, Shoji: "Synthetic curcumin analog EF31 inhibits the growth of head and neck squamous cell carcinoma xenografts." in: Integrative biology : quantitative biosciences from nano to macro, Vol. 4, Issue 6, pp. 633-40, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Fan, Söder, Oehler, Fundel, Aigner: "Activation of interleukin-1 signaling cascades in normal and osteoarthritic articular cartilage." in: The American journal of pathology, Vol. 171, Issue 3, pp. 938-46, 2007 (PubMed).

Kang, Kim, Kwon, Sok, Lee: "Enhancement of NF-kappaB expression and activity upon differentiation of human embryonic stem cell line SNUhES3." in: Stem cells and development, Vol. 16, Issue 4, pp. 615-23, 2007 (PubMed).

Starkey, Haidacher, LeJeune, Zhang, Tieu, Choudhary, Brasier, Denner, Tilton: "Diabetes-induced activation of canonical and noncanonical nuclear factor-kappaB pathways in renal cortex." in: Diabetes, Vol. 55, Issue 5, pp. 1252-9, 2006 (PubMed).

Popov, Patsenker, Bauer, Niedobitek, Schulze-Krebs, Schuppan: "Halofuginone induces matrix metalloproteinases in rat hepatic stellate cells via activation of p38 and NFkappaB." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15090-8, 2006 (PubMed).

Longpré, Garneau, Christen, Ramassamy: "Protection by EGb 761 against beta-amyloid-induced neurotoxicity: involvement of NF-kappaB, SIRT1, and MAPKs pathways and inhibition of amyloid fibril formation." in: Free radical biology & medicine, Vol. 41, Issue 12, pp. 1781-94, 2006 (PubMed).

Lee, Lee, Kim, Kwon: "Direct role of NF-kappaB activation in Toll-like receptor-triggered HLA-DRA expression." in: European journal of immunology, Vol. 36, Issue 5, pp. 1254-66, 2006 (PubMed).

Lee, Lee, Kwon, Kim: "Sp1-associated activation of macrophage inflammatory protein-2 promoter by CpG-oligodeoxynucleotide and lipopolysaccharide." in: Cellular and molecular life sciences : CMLS, Vol. 62, Issue 2, pp. 188-98, 2005 (PubMed).

Joo, Won, Lee: "Potential role of ursodeoxycholic acid in suppression of nuclear factor kappa B in microglial cell line (BV-2)." in: Archives of pharmacal research, Vol. 27, Issue 9, pp. 954-60, 2004 (PubMed).