Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (C-Term) antibody

Details for Product No. ABIN151761
Antigen
  • Ph-4
  • RGD1311848
  • EGLN4
  • HIFPH4
  • P4H-TM
  • PH-4
  • PH4
  • PHD4
  • 4933406E20Rik
  • AI853847
  • BB128974
  • prolyl 4-hydroxylase, transmembrane
  • prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
  • shisa family member 5
  • P4htm
  • P4HTM
  • SHISA5
Epitope
C-Term
4
4
4
3
1
1
1
Reactivity
Human, Rat (Rattus)
64
49
38
4
4
4
4
3
3
2
2
1
Host
Rabbit
63
2
Clonality
Polyclonal
Conjugate
Un-conjugated
4
3
3
3
3
3
3
3
3
2
2
2
2
2
Application
Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
27
26
6
6
4
3
1
Options
Immunogen The epitope recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 502) of human PHD4/HIF Prolyl Hydroxylase 4 using the numbering given in entry NP_808808.1 (GeneID 54681).
No Cross-Reactivity Mouse (Murine)
Purification Immunogen affinity purified
Antigen
Alternative Name PHD4/HIF Prolyl Hydroxylase 4 (P4HTM Antibody Abstract)
Background Gene Symbol: P4HTM
Molecular Weight Theoretical MW: 50 kDa
Gene ID 54681
NCBI Accession NP_808808
Application Notes Western Blot 1:500-1:2500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Electron MicroscopyThis PHD4/HIF Prolyl Hydroxylase 4 antibody is useful for Western Blot where a band is seen at ~50 kDa. Use in Immunocytochemistry/immunofluorescence, Immunohistochemistry-Paraffin and Electron Microscopy reported in scientific literature (PMID 17003483) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Image no. 1 for anti-Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (C-Term) antibody (ABIN151761) Western Blot analysis of human PHD4, using ABIN151761. Samples: whole cell lysate fro...
Image no. 2 for anti-Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (C-Term) antibody (ABIN151761) Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [ABIN151761] - Whole cell lysate...
Product cited in: Klotzsche-von Ameln, Prade, Grosser, Kettelhake, Rezaei, Chavakis, Flamme, Wielockx, Breier: "PHD4 stimulates tumor angiogenesis in osteosarcoma cells via TGF-α." in: Molecular cancer research : MCR, Vol. 11, Issue 11, pp. 1337-48, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Henze, Riedel, Diem, Wenner, Flamme, Pouyseggur, Plate, Acker: "Prolyl hydroxylases 2 and 3 act in gliomas as protective negative feedback regulators of hypoxia-inducible factors." in: Cancer research, Vol. 70, Issue 1, pp. 357-66, 2010 (PubMed).

Khan, Michalopoulos, Stolz: "Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation." in: The American journal of pathology, Vol. 169, Issue 4, pp. 1251-69, 2006 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunoelectron Microscopy,Immunofluorescence

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