RPE65 antibody (Retinal Pigment Epithelium-Specific Protein 65kDa)

Details for Product anti-RPE65 Antibody No. ABIN151819
Antigen
  • LCA2
  • RP20
  • mRPE65
  • rd12
  • sRPE65
  • 65kDa
  • A930029L06Rik
  • Mord1
  • DRPE65
  • RPE65
  • anon-WO0148162.1
  • anon-WO0148163.1
  • beta-diox
  • DmelCG9347
  • CG9347
  • MGC52887
  • MGC154296
  • MGC85437
  • rpe65
  • RPE56c
  • si:ch211-198n5.12
  • RPE56a
  • rpepa
  • zgc:73213
  • RPE65, retinoid isomerohydrolase
  • retinal pigment epithelium 65
  • neither inactivation nor afterpotential B
  • retinal pigment epithelium-specific protein 65kDa S homeolog
  • retinal pigment epithelium-specific protein 65kDa L homeolog
  • RPE65 homolog
  • retinal pigment epithelium-specific protein 65kDa
  • retinal pigment epithelium-specific protein 65c
  • retinal pigment epithelium-specific protein 65a
  • RPE65
  • Rpe65
  • ninaB
  • rpe65.S
  • rpe65.L
  • rpe65
  • rpe65c
  • rpe65a
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
50
45
37
13
4
3
1
1
1
1
1
Host
Mouse
45
10
9
Clonality (Clone)
Monoclonal ()
Conjugate
This RPE65 antibody is un-conjugated
6
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
41
21
13
9
6
5
3
3
2
1
1
Options
Immunogen Bovine RPE65 microsomal membrane proteins. [UniProt# Q28175]
Clone 401-8B11-3D9
Isotype IgG1 kappa
Specificity This antibody is specific for RPE65.
Purification Protein G purified
Plasmids, Primers & others Plasmids, Primers & others RPE65 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name RPE65 (RPE65 Antibody Abstract)
Background Gene Symbol: RPE65
Molecular Weight Theoretical MW: 65 kDa
Gene ID 6121
UniProt Q28175
Application Notes Western Blot 1:500 - 1:1000, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:250-1:500, Immunohistochemistry-Frozen 1:250-1:500, CyTOF-readyFor Western blot, this antibody has been validated in lysates of bovine RPE membrane and COS7 cells transfected with human RPE65, and in both samples, the antibody recognized a band at ~65 kDa, representing RPE65 protein. Note:- Hamel et al. have reported that in cultured bovine RPE cells, the levels of RPE65 are undetectable in WB after day 14, however, the mRNA levels are detectable by Northern for at least 7 weeks (PMID: 8340400). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for RPE65 Antibody Western Blot
1. Bovine RPE membrane (5ug) and recombinant human RPE65 (~20ug ) expressed in COS7 cells were loaded on a NuPAGE Novex Bis-Tris 10 %gel along with SeeBlue Plus2 marker.
. Run in NuPAGE MOPS electrophoresis buffer (Invitrogen). 3 Samples were probed with NB 100-355 RPE65 antibody (1:5,000 ) followed by alkaline phosphatase-conjugated goat anti-mouse IgG incubation.
. Visualized by NBT/BCIP substrate.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65) antibody (ABIN151819) RPE65 detected in bovine and human samples. Lane 1: bovine RPE membrane, lane 2: reco...
Immunofluorescence (IF) image for anti-Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65) antibody (ABIN151819) Immunohistochemical staining of RPE65 in mouse retina tissue using ABIN151819.
Immunofluorescence (IF) image for anti-Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65) antibody (ABIN151819) Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [ABIN151819] - ...
Immunocytochemistry (ICC) image for anti-Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65) antibody (ABIN151819) Immunocytochemistry: RPE65 Antibody (401.8B11.3D9) - ARPE-19 monolayer on glass subs...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65) antibody (ABIN151819) Immunohistochemistry-Paraffin: RPE65 Antibody (401.8B11.3D9) - IHC analysis of forma...
Immunofluorescence (IF) image for anti-Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65) antibody (ABIN151819) Immunofluorescence: RPE65 Antibody (401.8B11.3D9) - Zebrafish brain ventricular area...
Product cited in: Cho, Park, Lee, Nah, Park, Yang, Madrakhimov, Lyu, Park: "The role of Wnt/β-catenin signaling in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium after laser photocoagulation." in: Lasers in medical science, Vol. 34, Issue 3, pp. 571-581, 2019 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Cai, Gong, Abriola, Hoyer, Nyscf Global Stem Cell Array Team, Noggle, Paull, Del Priore, Fields: "High-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD." in: Experimental eye research, Vol. 185, pp. 107641, 2019 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Gao, Liao, Chen, Liao, He, Chen, Ma, Liu, Wu: "Conversion of all-trans-retinal into all-trans-retinal dimer reflects an alternative metabolic/antidotal pathway of all-trans-retinal in the retina." in: The Journal of biological chemistry, Vol. 293, Issue 37, pp. 14507-14519, 2019 (PubMed).

Sun, Huang, Liang, Li, Shen, She, Feng, Luo, Liu, Sun: "Cooperation of Rel family members in regulating Aβ1-40-mediated pro-inflammatory cytokine secretion by retinal pigment epithelial cells." in: Cell death & disease, Vol. 8, Issue 10, pp. e3115, 2018 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).

Bhutto, Ogura, Baldeosingh, McLeod, Lutty, Edwards: "An Acute Injury Model for the Phenotypic Characteristics of Geographic Atrophy." in: Investigative ophthalmology & visual science, Vol. 59, Issue 4, pp. AMD143-AMD151, 2018 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Rat (Rattus)).

Llonch, Carido, Ader: "Organoid technology for retinal repair." in: Developmental biology, Vol. 433, Issue 2, pp. 132-143, 2018 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Kaur, Tan, Rathnasamy, La Cunza, Germer, Toops, Fernandes, Blenkinsop, Lakkaraju: "Aberrant early endosome biogenesis mediates complement activation in the retinal pigment epithelium in models of macular degeneration." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 36, pp. 9014-9019, 2018 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Liao, Yan, Zeng, Wang, Qiu, Xu, Zheng: "Insulin-like growth factor-1 activates PI3K/Akt signalling to protect human retinal pigment epithelial cells from amiodarone-induced oxidative injury." in: British journal of pharmacology, Vol. 175, Issue 1, pp. 125-139, 2018 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Rat (Rattus)).

Chae, Rho, Shin, Lyu, Kang: "Effects of Ranibizumab, Bevacizumab, and Aflibercept on Senescent Retinal Pigment Epithelial Cells." in: Korean journal of ophthalmology : KJO, Vol. 32, Issue 4, pp. 328-338, 2018 (PubMed). Method employed by authors: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Zheng, Meng, Wang, Yan, Little, Deng, Lin: "IGF-1-Mediated Survival from Induced Death of Human Primary Cultured Retinal Pigment Epithelial Cells Is Mediated by an Akt-Dependent Signaling Pathway." in: Molecular neurobiology, 2017 (PubMed). (Sample species: Human).

Chowers, Cohen, Marks-Ohana, Stika, Eijzenberg, Banin, Obolensky: "Course of Sodium Iodate-Induced Retinal Degeneration in Albino and Pigmented Mice." in: Investigative ophthalmology & visual science, Vol. 58, Issue 4, pp. 2239-2249, 2017 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Steinfeld, Steinfeld, Bausch, Coronato, Hampel, Depner, Layer, Vogel-Höpker: "BMP-induced reprogramming of the neural retina into retinal pigment epithelium requires Wnt signalling." in: Biology open, Vol. 6, Issue 7, pp. 979-992, 2017 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Chicken).

Inoue, Hasegawa, Yamada, Date, Mizutani, Nakata, Akamatsu: "Lignin Induces ES Cells to Differentiate into Neuroectodermal Cells through Mediation of the Wnt Signaling Pathway." in: PLoS ONE, Vol. 8, Issue 6, pp. e66376, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Cashman, Gracias, Adhi, Kumar-Singh: "Adenovirus-mediated delivery of Factor H attenuates complement C3 induced pathology in the murine retina: a potential gene therapy for age-related macular degeneration." in: The journal of gene medicine, Vol. 17, Issue 10-12, pp. 229-43, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Parker, Mitrousis, Shoichet: "Hydrogel for Simultaneous Tunable Growth Factor Delivery and Enhanced Viability of Encapsulated Cells in Vitro." in: Biomacromolecules, Vol. 17, Issue 2, pp. 476-84, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Chen, Rajapakse, Fraczek, Luo, Forrester, Xu: "Retinal pigment epithelial cell multinucleation in the aging eye - a mechanism to repair damage and maintain homoeostasis." in: Aging cell, Vol. 15, Issue 3, pp. 436-45, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Ayala-Peña, Pilotti, Volonté, Rotstein, Politi, German: "Protective effects of retinoid x receptors on retina pigment epithelium cells." in: Biochimica et biophysica acta, Vol. 1863, Issue 6 Pt A, pp. 1134-45, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Iwabe, Ying, Aguirre, Beltran: "Assessment of visual function and retinal structure following acute light exposure in the light sensitive T4R rhodopsin mutant dog." in: Experimental eye research, Vol. 146, pp. 341-53, 2016 (PubMed). (Sample species: Dog (Canine)). Further details: Immunohistochemistry (Frozen Sections)

Zhou, Rivera, Bhosle, Lahaie, Shao, Tahiri, Zhu, Polosa, Dorfman, Beaudry-Richard, Costantino, Lodygensky, Lachapelle, Chemtob: "Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1β." in: The American journal of pathology, Vol. 186, Issue 12, pp. 3100-3116, 2016 (PubMed). Method employed by authors: Flow Cytometry (FACS)

Di Lauro, Rodriguez-Crespo, Gayoso, Garcia-Gutierrez, Pastor, Srivastava, Fernandez-Bueno: "A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells." in: Molecular vision, Vol. 22, pp. 243-53, 2016 (PubMed).

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