FLT1 antibody (Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor)) (Internal Region)

Details for Product anti-FLT1 Antibody No. ABIN151960, Supplier: Log in to see
Antigen
  • FLT
  • FLT-1
  • VEGFR-1
  • VEGFR1
  • FLT1
  • flt1b
  • sFlt1
  • AI323757
  • Flt-1
  • fms related tyrosine kinase 1
  • FMS-related tyrosine kinase 1
  • vascular endothelial growth factor receptor 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
  • FMS-like tyrosine kinase 1
  • FLT1
  • Flt1
  • CpipJ_CPIJ012087
  • flt1
Alternatives
anti-Human FLT1 antibody for Enzyme Immunoassay
12 anti-Human FLT1 antibody for Enzyme Immunoassay
52 anti-Human FLT1 antibody for Immunoprecipitation
243 anti-Human FLT1 antibody for Western Blotting
6 anti-Mouse (Murine) FLT1 antibody for Enzyme Immunoassay
12 anti-Mouse (Murine) FLT1 antibody for Immunoprecipitation
98 anti-Mouse (Murine) FLT1 antibody for Western Blotting
88 anti-Human FLT1 antibody for Flow Cytometry
99 anti-Human FLT1 antibody for Immunofluorescence
21 anti-Mouse (Murine) FLT1 antibody for Immunofluorescence
77 anti-Human FLT1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
23 anti-Mouse (Murine) FLT1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
22 anti-Rat (Rattus) FLT1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
71 anti-Rat (Rattus) FLT1 antibody for Western Blotting
103 anti-Human FLT1 antibody for Immunohistochemistry
172 anti-Human FLT1 antibody for ELISA
54 anti-Mouse (Murine) FLT1 antibody for ELISA
2 anti-Mouse (Murine) FLT1 antibody for Neutralization
9 anti-Human FLT1 antibody for Immunohistochemistry (Frozen Sections)
32 anti-Human FLT1 antibody for Immunocytochemistry
17 anti-Mouse (Murine) FLT1 antibody for Flow Cytometry
16 anti-Mouse (Murine) FLT1 antibody for Immunocytochemistry
38 anti-Mouse (Murine) FLT1 antibody for Immunohistochemistry
1 anti-Mouse (Murine) FLT1 antibody for Immunohistochemistry (Frozen Sections)
5 anti-Rat (Rattus) FLT1 antibody for Flow Cytometry
5 anti-Rat (Rattus) FLT1 antibody for Immunocytochemistry
6 anti-Rat (Rattus) FLT1 antibody for Immunofluorescence
42 anti-Rat (Rattus) FLT1 antibody for Immunohistochemistry
1 anti-Rat (Rattus) FLT1 antibody for Immunohistochemistry (Frozen Sections)
5 anti-Rat (Rattus) FLT1 antibody for Immunoprecipitation
45 anti-Rat (Rattus) FLT1 antibody for ELISA
1 anti-Human FLT1 antibody for Proximity Ligation Assay
1 anti-Human FLT1 antibody for Dot Blot
13 anti-Human FLT1 antibody for Immunofluorescence (Paraffin-embedded Sections)
13 anti-Mouse (Murine) FLT1 antibody for Immunofluorescence (Paraffin-embedded Sections)
13 anti-Rat (Rattus) FLT1 antibody for Immunofluorescence (Paraffin-embedded Sections)
3 anti-Wild boar (Sus scrofa) FLT1 antibody for Western Blotting
2 anti-Wild boar (Sus scrofa) FLT1 antibody for Immunocytochemistry
1 anti-Wild boar (Sus scrofa) FLT1 antibody for ELISA
17 anti-Pig (Porcine) FLT1 antibody for Western Blotting
2 anti-Pig (Porcine) FLT1 antibody for Immunocytochemistry
1 anti-Pig (Porcine) FLT1 antibody for ELISA
3 anti-Wild boar (Sus scrofa) FLT1 antibody for Immunohistochemistry
9 anti-Pig (Porcine) FLT1 antibody for Immunohistochemistry
1 anti-Human FLT1 antibody for Immunochromatography
1 anti-Cow (Bovine) FLT1 antibody for Western Blotting
2 anti-Dog (Canine) FLT1 antibody for Western Blotting
2 anti-Guinea Pig FLT1 antibody for Western Blotting
2 anti-Horse (Equine) FLT1 antibody for Western Blotting
1 anti-Gibbon FLT1 antibody for Immunohistochemistry
2 anti-Gorilla FLT1 antibody for Immunohistochemistry
2 anti-Chicken FLT1 antibody for Western Blotting
3 anti-Monkey FLT1 antibody for Western Blotting
2 anti-Rabbit FLT1 antibody for Western Blotting
2 anti-Sheep (Ovine) FLT1 antibody for Western Blotting
14 anti-Zebrafish (Danio rerio) FLT1 antibody for ELISA
7 anti-Human FLT1 antibody for Functional Studies
1 anti-Mouse (Murine) FLT1 antibody for ELISA (Detection)
2 anti-Human FLT1 antibody for Cytometry by Time of Flight
16 anti-Zebrafish (Danio rerio) FLT1 antibody for Immunohistochemistry
13 anti-Zebrafish (Danio rerio) FLT1 antibody for Western Blotting
1 anti-Human FLT1 antibody for Blocking Peptide
1 anti-Human FLT1 antibody for Neutralization
1 anti-Mouse (Murine) FLT1 antibody for Blocking Peptide
2 anti-Pig (Porcine) FLT1 antibody for Immunoprecipitation
1 anti-Human FLT1 antibody for Blocking Reagent
1 anti-Mouse (Murine) FLT1 antibody for Blocking Reagent
1 anti-Mouse (Murine) FLT1 antibody for ELISA (Capture)
4 anti-Mouse (Murine) FLT1 antibody for Immunofluorescence (fixed cells)
8 anti-Rat (Rattus) FLT1 antibody for Immunofluorescence (fixed cells)
6 anti-Pig (Porcine) FLT1 antibody for Immunofluorescence (fixed cells)
1 anti-Bat FLT1 antibody for Western Blotting
1 anti-Goat FLT1 antibody for Western Blotting
1 anti-Monkey FLT1 antibody for ELISA
1 anti-Monkey FLT1 antibody for Immunohistochemistry
1 anti-Monkey FLT1 antibody for Immunoprecipitation
1 anti-Mouse (Murine) FLT1 antibody for Cytometry by Time of Flight
6 anti-Human FLT1 antibody for Immunofluorescence (fixed cells)
2 anti-Zebrafish (Danio rerio) FLT1 antibody for Immunocytochemistry
2 anti-Zebrafish (Danio rerio) FLT1 antibody for Immunofluorescence
Epitope
Internal Region, AA 800-900
26
16
16
12
12
8
8
7
6
6
6
5
5
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
344
120
113
17
3
3
2
2
2
2
2
2
2
1
1
1
1
Host
Rabbit
238
155
13
6
3
1
Clonality
Polyclonal
Conjugate
This FLT1 antibody is un-conjugated
28
17
7
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
243
138
101
101
73
54
46
22
13
13
12
7
7
3
3
2
2
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen A synthetic peptide made to an internal region of the mouse VEGF Receptor 1 protein (between residues 800-900) [UniProt# P35969]. There is 87% identity between the immunogen used for this production and the VEGF Receptor 2 protein.
Specificity This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.
Purification Immunogen affinity purified
Plasmids, Primers & others Plasmids, Primers & others FLT1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name VEGFR1/Flt-1 (FLT1 Antibody Abstract)
Background Gene Symbol: FLT1
Gene ID 2321
UniProt P35969
Pathways RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Application Notes Western Blot 1:100-1:2000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry-ParaffinThis VEGF Receptor 1 antibody is useful for Western blot, where a band is seen at ~180-230 kDa (the theoretical molecular weight of mouse VEGFR1 is ~150 kDa and human VEGFR1 is ~151 kDa). The difference in MWs is likely due to glycosylation or other post translational modifications. With CSF-1R/VEGFR2 chimera transfected lysates a doublet is seen at ~150 kDa representing VEGFR-2. An unknown band is also seen at ~50 kDa. Optimal working dilutions should be determined by the investigator.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol Protocol specific for VEGF Receptor 1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 50 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 0.5 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-VEGFR1 primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 800-900), (Internal Region) antibody (ABIN151960) Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.
Immunofluorescence (IF) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 800-900), (Internal Region) antibody (ABIN151960) Immunocytochemistry/Immunofluorescence: VEGF R1/Flt-1 Antibody [ABIN151960] - VEGF R1...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 800-900), (Internal Region) antibody (ABIN151960) Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody - IHC analysis of a formalin-fi...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 800-900), (Internal Region) antibody (ABIN151960) Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody - IHC analysis of a formalin-fi...
Product cited in: Su: "Tanshinone IIA can inhibit MiaPaCa‑2 human pancreatic cancer cells by dual blockade of the Ras/Raf/MEK/ERK and PI3K/AKT/mTOR pathways." in: Oncology reports, Vol. 40, Issue 5, pp. 3102-3111, 2018 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Puglisi, Puppin, Pegolo, Andreetta, Pascoletti, DAurizio, Pandolfi, Fasola, Piga, Damante, Di Loreto: "Expression of periostin in human breast cancer." in: Journal of clinical pathology, Vol. 61, Issue 4, pp. 494-8, 2008 (PubMed).

Ptaszynska, Pendrak, Bandle, Stracke, Roberts: "Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells." in: Molecular cancer research : MCR, Vol. 6, Issue 3, pp. 352-63, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting

Takahashi, Hattori, Iwamatsu, Takizawa, Shibuya: "A novel snake venom vascular endothelial growth factor (VEGF) predominantly induces vascular permeability through preferential signaling via VEGF receptor-1." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46304-14, 2004 (PubMed).

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000 (PubMed).

Background publications Gluzman-Poltorak, Cohen, Shibuya, Neufeld: "Vascular endothelial growth factor receptor-1 and neuropilin-2 form complexes." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 18688-94, 2001 (PubMed).

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