MMP2 antibody (Matrix Metalloproteinase 2)

Details for Product anti-MMP2 Antibody No. ABIN152258, Supplier: Log in to see
Antigen
  • 2-MMP
  • CG1794
  • DM2-MMP
  • Dm2-MMP
  • Dmel\\CG1794
  • MMP2
  • anon-WO0118547.84
  • dm-2MMP
  • dmmp2
  • l(2)02353
  • mmp2
  • wu:fa99h12
  • wu:fk89d01
  • fgmmp-2
  • Mmp-2
  • LOC100135793
  • Clg4a
  • GelA
  • MMP-2
  • CLG4
  • CLG4A
  • MMP-II
  • MONA
  • TBE-1
  • matrix metallopeptidase 2
  • Matrix metalloproteinase 2
  • matrix metalloproteinase 2
  • matrix metalloproteinase-2
  • MMP2
  • Mmp2
  • mmp2
  • LOC657982
  • LOC100135793
Alternatives
anti-Rat (Rattus) MMP2 antibody for Immunoprecipitation
Reactivity
Human, Mouse (Murine), Rat (Rattus)
397
183
173
29
16
15
13
11
7
6
6
5
4
2
2
2
2
2
1
1
1
Host
Mouse
250
176
2
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This MMP2 antibody is un-conjugated
20
17
15
8
8
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
326
150
111
106
90
72
63
60
53
39
19
6
5
2
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Activated recombinant human MMP2. [UniProt# P08253]
Clone 8B4
Isotype IgG1 kappa
Specificity This is specific for pro and active MMP2.
Purification Protein G purified
Plasmids, Primers & others Plasmids, Primers & others MMP2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name MMP-2 (MMP2 Antibody Abstract)
Background Gene Symbol: MMP2
Gene ID 4313
UniProt P08253
Pathways Activation of Innate immune Response
Application Notes Western Blot 1-2 μg/mL, ELISA 0.03 μg/mL, Immunohistochemistry 2 μg/mL, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1-2 μg/100-500 μg total protein, Immunohistochemistry-Paraffin 2 μg/mL, Immunohistochemistry-Frozen 2 μg/mLThis MMP2 antibody (8B4) is useful for Western Blot, Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. In WB, a band is seen at 70 and 62 kDa representing the MMP2 band-pair.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol IHC (Paraffin) Protocol Specific for MMP2 Antibody For IHC on Paraffin-Embedded Tissue Sections:
1. Deparaffinize in xylene. 3 changes at 10 min each.
. Hydrate in 100 % EtOH for 5 min (2X).
. Quench endogenous peroxidase. 30 min in 1 % H2O2 in methanol. (Make fresh each time: 6 mL 30 % H2O2+ 194 mL methanol).
. Hydrate to H2O in graded alcohols. a. 5 min in 95 %.b. 5 min in 70 %c. 5 min in distilled H2O.
. Perform antigen retrieval (Antigen Retrieval, Dako #S1700) with heating as described by manufacturer. Fill plastic Coplin jar with 50 mL Antigen Retrieval buffer. Add slides to jar when temperature reaches 95-99C start timing. Heat for 30 min. After heating, let slides cool in jar for 15 min.
. Wash slides in PBS for 5 min (2x).
. Infiltrate sections with PBS + 0.5 % Triton X100 for 10 min, then rinse twice in PBS.
. Block for 1 hr. at room temp in Blocking buffer- (PBS + 10 % goat serum).
. Aspirate blocking buffer. Apply MMP2/8B4 IgG diluted to 5 µg/ml in Blocking buffer. Incubate overnight at 4C (or >2 hr at 37C).
. Rinse 3X in PBS for 10 min each.
. Apply 2 degrees antibody. Dilute biotin conjugated goat anti-mouse 1:500 (Dako # E0433) in Blocking buffer.
. Incubate for 2 hr. at room temperature.
. Rinse 3X in PBS for 10 min each.
. Prepare Avidin-biotin-peroxidase by combining each component at 1:50 in PBS + 0.1 % Triton X
. Gently mix and react for 30 min. Prior to use, dilute the complexed mixture 1:5 with PBS + 0.1 % BSA. Apply to sections and incubate for 2 hr at room temp.
. Rinse in 3X in PBS for 15 min each.
. Develop with DAB. Dissolved 1.5 mg diaminobenzidine-(HCl)4 in 3 mL of PBS, then add 2 µL of 30 % hydrogen peroxide. Filter through a 0.2 um filter immediately before use. Develop for 15 min.
. Rinse in water for 5 min. Counterstain for nuclei (e.g, hematoxylin) as desired.
. Dehydrate through graded ethanol, clear in 100 % ethanol and xylene (1:1 solution) and then 100 % xylene. Coverslip with Permount.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Matrix Metalloproteinase 2 (MMP2) antibody (ABIN152258) Detection of MMP-2 staining in formalin-fixed paraffin-embedded human ovary tissue us...
Western Blotting (WB) image for anti-Matrix Metalloproteinase 2 (MMP2) antibody (ABIN152258) Western Blot: MMP2 Antibody (8B4) [ABIN152258] - Western blot analysis of MMP2 expres...
Product cited in: Erikson, Valente, Mummidi, Kandikattu, DeMarco, Bender, Fay, Siebenlist, Chandrasekar: "Targeting TRAF3IP2 by Genetic and Interventional Approaches Inhibits Ischemia/Reperfusion-induced Myocardial Injury and Adverse Remodeling." in: The Journal of biological chemistry, Vol. 292, Issue 6, pp. 2345-2358, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Sakamuri, Valente, Siddesha, Delafontaine, Siebenlist, Gardner, Bysani: "TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and fibrosis." in: Molecular and cellular endocrinology, Vol. 429, pp. 84-92, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Siddesha, Valente, Yoshida, Sakamuri, Delafontaine, Iba, Noda, Chandrasekar: "Docosahexaenoic acid reverses angiotensin II-induced RECK suppression and cardiac fibroblast migration." in: Cellular signalling, Vol. 26, Issue 5, pp. 933-41, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Huang, Cai, Lung, Leung, Burd: "A study of the combination of triamcinolone and 5-fluorouracil in modulating keloid fibroblasts in vitro." in: Journal of plastic, reconstructive & aesthetic surgery : JPRAS, Vol. 66, Issue 9, pp. e251-9, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

von Leithner, Ciurtin, Jeffery: "Microscopic mammalian retinal pigment epithelium lesions induce widespread proliferation with differences in magnitude between center and periphery." in: Molecular vision, Vol. 16, pp. 570-81, 2010 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Raaf, Noll, Cherifi, Samuel, Delcayre, Delabar, Benazzoug, Janel: "Myocardial fibrosis and TGFB expression in hyperhomocysteinemic rats." in: Molecular and cellular biochemistry, Vol. 347, Issue 1-2, pp. 63-70, 2010 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Locke, Royce, Wainewright, Samuel, Tang: "Comparison of airway remodeling in acute, subacute, and chronic models of allergic airways disease." in: American journal of respiratory cell and molecular biology, Vol. 36, Issue 5, pp. 625-32, 2007 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Qian, Dong, Kong, Ma, Fan, Jiang: "Suppression of type 1 Insulin-like growth factor receptor expression by small interfering RNA inhibits A549 human lung cancer cell invasion in vitro and metastasis in xenograft nude mice." in: Acta biochimica et biophysica Sinica, Vol. 39, Issue 2, pp. 137-47, 2007 (PubMed).

Ribeiro-Silva, Ribeiro do Vale, Zucoloto: "Vascular endothelial growth factor expression in the basal subtype of breast carcinoma." in: American journal of clinical pathology, Vol. 125, Issue 4, pp. 512-8, 2006 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Dong, Sato, Sakurada, Sagawa, Endo, Wu, Yamanaka, Horii, Kondo et al.: "Computed tomographic images reflect the biologic behavior of small lung adenocarcinoma: they correlate with cell proliferation, microvascularization, cell adhesion, degradation of extracellular ..." in: The Journal of thoracic and cardiovascular surgery, Vol. 130, Issue 3, pp. 733-9, 2005 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Wang, Yu, McCaughan, Gorrell: "Fibroblast activation protein increases apoptosis, cell adhesion, and migration by the LX-2 human stellate cell line." in: Hepatology (Baltimore, Md.), Vol. 42, Issue 4, pp. 935-45, 2005 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Alcorn, Fierer, Chojkier: "The acute-phase response protects mice from D-galactosamine sensitization to endotoxin and tumor necrosis factor-alpha." in: Hepatology (Baltimore, Md.), Vol. 15, Issue 1, pp. 122-9, 1992 (PubMed). (Sample species: Human). Further details: Western Blotting

Background publications Muir: "Differences in proliferation and invasion by normal, transformed and NF1 Schwann cell cultures are influenced by matrix metalloproteinase expression." in: Clinical & experimental metastasis, Vol. 13, Issue 4, pp. 303-14, 1995 (PubMed).

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