Peroxisome Proliferator-Activated Receptor alpha (PPARA) antibody

Details for Product No. ABIN152696
Antigen
  • NR1C1
  • PPAR
  • PPARalpha
  • hPPAR
  • LOC100136415
  • PPARALPHA
  • nr1c1
  • xPPARalpha
  • xppara
  • 4933429D07Rik
  • AW742785
  • Nr1c1
  • PPAR-alpha
  • Ppar
  • peroxisome proliferator activated receptor alpha
  • peroxisome proliferator-activated receptor alpha
  • peroxisome proliferator activated receptor alpha L homeolog
  • PPARA
  • LOC100136415
  • ppara.L
  • Ppara
  • ppara
Alternatives
anti-Golden Syrian Hamster Peroxisome Proliferator-Activated Receptor alpha antibody for Immunohistochemistry
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
204
155
110
12
11
10
9
9
7
6
6
4
4
3
2
2
1
1
1
Host
Mouse
187
51
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
10
5
5
5
5
5
5
5
5
3
2
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
182
63
54
46
45
26
26
19
10
9
6
4
4
2
2
1
1
Options
Immunogen Purified recombinant PPAR alpha protein.
Clone 3B6-PPAR
Isotype IgG2b
Specificity PPAR alpha (3B6/PPAR)
Purification Immunogen affinity purified
Antigen
Alternative Name PPAR alpha/nr1c1 (PPARA Antibody Abstract)
Background Gene Symbol: PPARA
Gene ID 5465
UniProt Q07869
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C
Application Notes Western Blot 2 - 5 μg/mL, Chromatin Immunoprecipitation 1:10 - 1:500, Flow Cytometry 1 μg/ 10^6 cells, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 2 μg/mL, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:200, Gel Super Shift Assays 1:1 - 1:100May be useful in ChIP. Rabbit-IP reported in publication, see Sumanasekera WK et al. Rat-IHC reported in publication, see Crespillo A et al.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Flow Cytometry (FACS) image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) antibody (ABIN152696) Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [ABIN152696] - Analysis of PPAR ...
Flow Cytometry (FACS) image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) antibody (ABIN152696) Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [ABIN152696] - Analysis of PPAR ...
Western Blotting (WB) image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) antibody (ABIN152696) Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [ABIN152696] - 293T cell lysate. I...
Flow Cytometry (FACS) image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) antibody (ABIN152696) Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [ABIN152696] - Analysis of PPAR ...
Flow Cytometry (FACS) image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) antibody (ABIN152696) Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - An intracellular stain was pe...
Western Blotting (WB) image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) antibody (ABIN152696) Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - Analysis of 25 ug of Hela (Lane...
Product cited in: Kumar, Rani, Karns, Gandhi: "Augmenter of liver regeneration protein deficiency promotes hepatic steatosis by inducing oxidative stress and microRNA-540 expression." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, Issue 3, pp. 3825-3840, 2019 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Li, He, Xiao, Liu, Wang, Zhang: "Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 45, Issue 5, pp. 2021-2030, 2018 (PubMed).

Sun, Zhang, Zhong, Sun, Zhou: "Pharmacological inhibition of NOX4 ameliorates alcohol-induced liver injury in mice through improving oxidative stress and mitochondrial function." in: Biochimica et biophysica acta. General subjects, Vol. 1861, Issue 1 Pt A, pp. 2912-2921, 2017 (PubMed).

Tatone, Benedetti, Vitti, Di Emidio, Ciriminna, Vento, Cela, Borzì, Carta, Lispi, Cimini, Artini: "Modulating Intrafollicular Hormonal Milieu in Controlled Ovarian Stimulation: Insights From PPAR Expression in Human Granulosa Cells." in: Journal of cellular physiology, Vol. 231, Issue 4, pp. 908-14, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Rotondi, Modarelli, Oliva, Rostomyan, Sanita, Ventura, Daly, Esposito, Angelucci, Arcella, Giangaspero, Beckers, Jaffrain-Rea et al.: "Expression of Peroxisome Proliferator-Activated Receptor alpha (PPARα) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate ..." in: Molecular and cellular endocrinology, Vol. 426, pp. 61-72, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Gao, Xu, Li: "The expression and role of peroxisome proliferator-activated receptor α in atherosclerosis." in: Molecular medicine reports, Vol. 7, Issue 3, pp. 862-8, 2013 (PubMed). (Sample species: Rabbit).

Crespillo, Suárez, Bermúdez-Silva, Rivera, Vida, Alonso, Palomino, Lucena, Serrano, Pérez-Martín, Macias, Fernández-Llébrez, Rodríguez de Fonseca: "Expression of the cannabinoid system in muscle: effects of a high-fat diet and CB1 receptor blockade." in: The Biochemical journal, Vol. 433, Issue 1, pp. 175-85, 2010 (PubMed). Further details: Western Blotting,Immunohistochemistry

Liu, Heymann, Moatamed, Schultz, Sobel, Brent: "A mutant thyroid hormone receptor alpha antagonizes peroxisome proliferator-activated receptor alpha signaling in vivo and impairs fatty acid oxidation." in: Endocrinology, Vol. 148, Issue 3, pp. 1206-17, 2007 (PubMed). Further details: Western Blotting

Alwayn, Andersson, Lee, Arsenault, Bistrian, Gura, Nose, Zauscher, Moses, Puder: "Inhibition of matrix metalloproteinases increases PPAR-alpha and IL-6 and prevents dietary-induced hepatic steatosis and injury in a murine model." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 291, Issue 6, pp. G1011-9, 2006 (PubMed). Further details: Immunoprecipitation

Sumanasekera, Tien, Turpey, Vanden Heuvel, Perdew: "Evidence that peroxisome proliferator-activated receptor alpha is complexed with the 90-kDa heat shock protein and the hepatitis virus B X-associated protein 2." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 4467-73, 2003 (PubMed). Further details: Immunoprecipitation

Did you look for something else?